TARGATT Embryos

Using our novel TARGATT system, a gene of interest can be specifically inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and / or ubiquitous expression options are available.

Advantages of TARGATT technology:

1. Site-specific gene integration at a transcriptionally-active locus ensures high-level gene expression.
2. Integration happens at an intergenic region; no internal genes are disrupted.
3. The integrase system catalyzes a unidirectional integration event and results in a high efficiency in producing transgenic mice.
4. Gene integration at the same locus allows a precise comparison of the transgenics from one line to another.

TARGATT Kits

TARGATT Supporting Materials

Mouse Embryonic Fibroblasts (MEF)

●  CF-1
●  Neo-resistant
●  DR4
●  SNL (STO feeder cells)

SNL (STO feeder cells)

Cell Culture Products

●  Germline-tested & ESC-qualified FBS
●  Specialty Media
●  Basal Media (DMEM)
●  Stem Cell Growth and Differentiation Factors
●  ASC Small Molecules
●  3D Culture and Expansion System

Reprogramming

ESC/iPSC Characterization

  Pluripotency Protein Markers                   Stem Cell Gene Array
     

●  Pluripotency mRNA Markers
●  Components

ESC/iPSC Differentiation

●  Neural Differentiation
●  Dendritic Cell (DC) Generation

ES/iPS Cell Lines

Mouse ES Cell Lines                                 Human iPS Cells

Cell Depository

Primary Cell, cDNA, RNA (Normal)

●  Endocrine
●  Neural
●  Pulmonary
●  Digestive
●  Urinary
●  Reproductive
●  Skeletal Muscle
●  Hematopoietic
●  Integumentary
●  Cardiovascular
●  Miscellaneous

Primary Cell, cDNA, RNA (Disease)

●  Blood Disorders
●  Neurological Disorders
●  Degenerative Disorders
●  Metabolic Disorders
●  Cardiovascular Disorders
●  Congenital Disorders
●  Endocrine Disorders
●  Autoimmune Disorders
●  Genetic Disorders
●  Muscular Disorders
●  Oncogenic Disorders

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Read more about Targatt gene modification here

TARGATT Fast & Efficient Gene Modification in Human Cells. Make your cell line in 3 months!

• - Efficient insertion of any gene
• - Single-copy
• - Stable expression
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Based on our proprietary site-specific TARGATT technology for fast knock-in mouse (KI) generation, Applied StemCell Inc. (ASC) recently developed a TARGATT system specifically for introducing gene(s) of interest into mammalian cell lines. The unique advantage of this system is that any gene of interest can be inserted efficiently into a defined, transcriptionally-active locus with high gene expression in a single copy fashion.

Technical Details:

TARGATT technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses ?C31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by ?C31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.

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