| GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45
Fax 0032 16 50 90 45
GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
GENTAUR SRL IVA IT03841300167
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547, Yurok Circle
San Jose, CA 95123
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San Jose, CA. 96123
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Schweiz Züri +41435006251
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
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Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.
Source : E.coli
Catalog number : 04-GMPhuIL4-50 µg
Molecular weight : 15 KDa
Identity : rh IL-4 as measured by Elisa and Western Blot
Specific activity : > 13.10 6 units/mg compared to NIBSC standard . CT-h4.S cell proliferation assay.
650 000 IU /vial
Purity : > 98% as determined by SDS-PAGE and HPLC
Endotoxin content : < 0.1 EU/ µg of IL-4 (LAL test)
Host DNA : < 10 pg/ng protein
Sterility : sterile according to EP Test 2.6.1.
Stabiliser : contains Trehalose ; no preservative.
Physical state : freeze-dried
Stability : 12 months at – 20°C to – 80°C
3 months after reconstitution with water for injection ( as defined in EP Monograph 0169) when stored at -80°C
Reconstitution : use 500 µL water for injection in class A environment in order to keep the GMP grade .
Packaging unit : 50 µg protein (Lowry test)
GENTAUR rh IL-4 is manufactured in full compliance with cGMP in facilities approved by the Belgian Ministry of Health for the production and storage of medicinal products.
Use: GENTAUR rh IL-4 is not an approved medicinal product and cannot be injected as such to patients.
FOR EX VIVO CELL CULTURE AND FOR IN VITRO RESEARCH USE ONLY
Price: 483 Euro
37.5:1 Acrylamide to Bisacrylamide Stabilized Solution
Optimized for SDS-PAGE of Proteins (Laemmli gels)
Consistently Crystal Clear Gels, Zero Fluorescence
Stabilized for Long Shelf Life
Catalog Number: EC-890
ProtoGel forms an electrophoresis matrix that is ideal for the separation of proteins and polypeptides. ProtoGel is a stabilized, ready-to-use 30% (w/v) acrylamide/methylene bisacrylamide solution (37.5:1 ratio), manufactured from the highest quality materials, from which virtually all impurities have been removed. ProtoGel has zero acrylic acid content, eliminating the fixed charges that cause band streaking. Additionally, oxidation products such as aldehydes have been removed by a selective adsorption process. Aldehydes can attack proteins, altering the structure of the sample, and thus altering Rf values. With ProtoGel, you can trust that your results will be consistent one electrophoretic run to the next.
Storage: ProtoGel is stable for 24 months when stored tightly capped in a dark area at room temperature (20°C).
ProtoGel (30%) Protocol
Measure and Mix Solutions:
The volume of ProtoGel required for gel casting solutions of any volume and acrylamide concentration may be calculated from the following formula:
Vp =(X) (Vt)/30
Vp = Volume of 30% ProtoGel
X = % Monomer Desired in Gel
Vt = Total Volume of Gel Casting Solution
EXAMPLE: To make 100 ml of a 10% monomer gel, calculate the volume of Protogel to add as follows:
Vp = (10) (100)/30 = 33.3 ml
Initiate and Cast Gel:
For optimal results degas gel solution for 10 minutes under vacuum aspiration prior to innitiation with APS and TEMED. Add 1.0ml of 10% (w/v) ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Add 0.1 ml of TEMED for every 100ml of gel casting solution. Swirl gently to mix. Pour the solution into the gel casting cassette. The gel should begin to set in 10-20 minutes. To provide a sharp interface, overlay the gel with water saturated n-butanol during polymerization. Flush butanol away with water just before casting the stacking gel (below).
Cast Stacking Gel:
Use ProtoGel Stacking Buffer to make 10ml of a 4% stacking gel:
ProtoGel Stacking Buffer: 2.5ml
Deionized Water: 6.1ml
Add 0.05ml 10% Ammonium Persulfate and 0.01ml of TEMED. Gel will begin to set in 20 minutes.