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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

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      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
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    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

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    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

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    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

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    Monday, 18 March 2013 15:00

    Invented a pill to quickly sober

    gentaur-alcohol-pillsStudies of "drug" are still in a very early stage and tests are performed only on mice.

    Soon fans will be able to cup sober just one pill. Researchers made ​​a cocktail of alcohol metabolizing enzymes rapidly reduce the level of alcohol in the blood while drunk mouse forward "Daily Mail". 

    "Treatment", which compares with having millions of liver cells in your stomach, you may have a lasting impact on fans of spirits. Yangfeng Lou - professor of chemistry and biomolecular engineering, and Cheng Zhi - Professor of Biochemistry and Molecular Biology at the University of Southern California, injected mice with drunk nanocapsule containing two enzymes. One of the enzymes - oxidase comes as a by-product of hydrogen peroxide, which can be harmful. That's why he has to work with another enzyme to break down hydrogen peroxide. The study shows that drunken mice that were injected with these nanocapsule, sobered much faster than those who did not receive enzyme "treatment". The breakthrough is still in a very early stage and can not speak for its application in humans.

    But expert Lou argues that experience can lead to the invention of a new drug to act as an antidote to alcohol. He states that "drug" can be taken as a simple pill. "It's like you have millions of liver cells in your stomach that help you revise alcohol," said Professor Lu.

    Published in News
    Monday, 11 March 2013 16:35

    Pills may help you live to 150 years ...

    knockinout-mice-rat

    Scientists develop pill that can help people to live to 150 years, slowing the aging process, reported the British newspaper "Daily Telegraph".

    Drugs are synthetic versions of the organic chemical resveratrol, found in red wine, which is believed to slow aging, enhances the activity of the protein SIRT1. The pharmaceutical company GlaxoSmithKline (GSK) drug testing on people suffering from diabetes of the second type and psoriasis.

    Professor of Genetics at Harvard University David Sinclair said that aging can not "irreversible catastrophe." "We now investigate whether a benefit for people who are already healthy. Results are promising. We find that aging is irreversible disaster, as previously thought. Certain people may live up to 150 years, but it will not get there without further research" he said.

    Professor Sinclair said that the efforts of the activity of SIRT1 protein enhances cell activity, reducing their laziness. Previous experiments on mice, bees and flies that received the substances that enhance the activity of the protein showed that they live longer.

    Professor Sinclair allegedly performed experiments which showed that the substances based on resveratrol have a direct impact on health. Some scholars argue that the impact is real and experimental stealth. The results were published in the scientific journal "Science", wrote Thursday.

    Despite the controversy, some experiments that promise for diseases such as cancer, cardiovascular disease and heart failure, diabetes of the second type, Alzheimer's, Parkinson's, fatty liver disease, cataracts, osteoporosis, muscle atrophy, sleep disorders and inflammatory diseases such as psoriasis, arthritis and colitis.

    In the present study aimed to determine how these substances can help cure diseases associated with aging. But Professor Sinclair believes that in time will also examine the preventive effect. As statins are used today to prevent heart disease and stroke, as these substances can be used to delay many diseases, says the researcher.

    Published in News
    Monday, 11 March 2013 15:06

    Knock-in / Knock-out Mouse (7-months)

    We provide a high quality service for the generation of gene-targeted knock-in and knock-out mouse models. Our team has decades of experience in vector design, ES cell targeting and mouse handling. We will discuss your project needs with you and custom design your knockin/knockout mouse model. Our service includes consultation on available gene targeting vector design strategies and project assessment based on your model requirements.

    Service Milestones for the Generation of Knock-in/Knock-out Mouse Models

    • - Gene targeting vector construction and sequencing
    • - ES cell targeting, screening and expansion of positive ES cell clones
    • - Karyotyping of targeted ES cells
    • - Cre/FLP recombination (optional)
    • - Chimera production by blastocyst injection
    • - Breeding of chimeras for germline transmission
    • - Genotyping of offspring
    • - Transfer of heterozygous targeted mice to the customer
    • - Optional: breeding to generate homozygous mice

     

    Features

    Cost-effective

    • - Targatt has years of experience and an exceptionally high success rate in generating conventional gene-targeted mice with our proprietary mESC lines.

     

    High-Quality

    • - Our scientists have extensive experience in gene targeting and genetic mouse models. Our services always include a rigorous quality control with multiple check-points to ensure your projects are completed with highest accuracy. All mice are generated in and shipped from our California facility (NIH guidance certified).

    Please contact us with any questions regarding services not listed. We are happy to discuss your project with you and design customized strategies. We also offer Transgenic Mouse (Random Insertion) and Transgenic Rat Services (knockin, knockout).

    Testimony of Dr. Zhenheng Guo, PhD, Assistant Professor of Internal Medicine, Division of Endocrinology and Molecular Medicine, University of Kentucky

    Project: Germline-transmitted, conditional knock-out mice by tetraploid complementation

    "Overall, I am very satisfied with the quality of your service. After we received the mice, we did extensive studies to characterize them. All of the PCR reactions confirm the gene modifications are in the correct sites. We have also crossed mice with Cre mice to demonstrate that the Cre deleted the exon in heterozygous pups as expected. All data obtained showed that the mice were correctly targeted. In addition, I am very happy with the frequent communication with your scientists during the process. I highly recommend your services."

    Project types

    Express gene "X"

    Replace gene "X" with gene "Y"

    Delete gene "X"

    Random Integration

    Knockout/Knockin

    Knockout

    Knockin

     

    Conditional/inducible Knockout

    Conditional/inducible Knockin

       

    TARGATT

    (Site-Specific Knockin)

       

     

    Knock-in Mouse Models

    The introduction of a gene into a specified location of the mouse genome can be used for various applications. Mice can be homozygous or heterozygous for the inserted gene.

    • - Reporter genes (e.g. GFP, lacZ) are used for expression analysis of a gene of interest. The reporter gene is inserted into the gene of interested in-frame (non-disruptive), allowing for visualization of temporal and spatial gene expression pattern.
    • - Humanized disease models can be generated by inserting a human mutant gene or gene fragment into the corresponding mouse gene. The diseased human allel is thus transcribed in the appropriate genomic context and can be analyzed on behavioral, pathological, cellular and/or molecular level.
    • - DNA recombinases (CRE, FLP) can be inserted into a gene of interest. The celltype-specific expression of the recombinase then allows for gene inactivation in the desired tissue after crossing this knock-in mouse with a conditional knockout mouse.

     

    Knock-out Mouse Models

    The knockout technology is most commonly used for gene inactivation, either in a constitutive or conditional fashion.

    • - Constitutive knock-out mouse models are widely used to study gene function. The gene of interest is permanently inactivated in all cells of the animal. However, this non-conditional knock-out may cause lethality and is therefore not always recommended.
    • - Conditional knock-out models are inducible - the targeted gene is excised after crossing the mouse with a Cre-transgenic mouse line. Gene inactivation can be celltype-specific and/or chemically induced.

     

    Published in Targatt services
    Monday, 11 March 2013 14:50

    TARGATT Knock-in Mice (3-months)

    Targatt can create site-specific knock-in mice for you within 3 months. Using our novel TARGATT system, a gene of interest can be inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and/or ubiquitous expression options are available. Please contact us for a list of plasmid construct with reporter genes. 

    In addition to our full service we also offer individual service options to generate your TARGATT knock-in mouse.

    You can prepare the DNA using the TARGATT Kit and we do the pronuclear microinjection for you. Or we can prepare the DNA that you then inject after setting up your own TARGATT mouse colony.

    Please contact us to discuss your project plan for your fast & site-specific knock-in or TARGATT Knock-down Mouse services.

    * Prices are a general guideline for academic institutes and may vary.  Please inquire for quote.

    Advantages of TARGATT Technology:

    Site-specific DNA integration (knock-in) in transcriptionally-active locus

    • - Ensures robust gene expression
    • - Eliminates unpredictable phenotypes caused by random integration
    • - Allows for proper comparison of different transgenes

    Single-copy integration

    • - Eliminates repeat-induced gene silencing and genomic instability.

     

    Insertion of intact transgene

    • - Avoids truncated transgenes with unexpected phenotype

    High integration efficiency

    • Save time and costs!

     

    targatt-antibodies-gentaur-knockin-knockout-mouse-1

     

    Applied Stem Cell Inc.’s proprietary TARGATT Technology enables highly efficient site-specific gene integration in mammalian cells and animals. This technology uses PhiC31 integrase to insert any gene of interest into a specific docking site that was pre-engineered into an intergenic and transcriptionally active genomic locus. Our TARGATT Technology improves several aspects in the generation of transgenic cell lines and animals:

    1. High integration efficiency mediated by  PhiC31 integrase reduces time and cost

    2. Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high expression levels of the transgene

    3. Integration at intergenic region ensures that no internal genes are interrupted

    4. Single copy gene integration eliminates repeat-induced gene silencing and genomic instability

    5. Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.

    TARGATT Technology can be utilized for a variety of applications including reporter gene expression, gene knockdown and disease models.

    targatt-antibodies-gentaur-knockin-knockout-mouse-3

    Published in Targatt services
    Wednesday, 06 March 2013 11:03

    TARGATT Knockin Mice - Stem Cells Products

    targatt-stemcells-knockin-mouse-1TARGATT Embryos

    Using our novel TARGATT system, a gene of interest can be specifically inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and / or ubiquitous expression options are available.

     

    Advantages of TARGATT technology:

    1. Site-specific gene integration at a transcriptionally-active locus ensures high-level gene expression.
    2. Integration happens at an intergenic region; no internal genes are disrupted.
    3. The integrase system catalyzes a unidirectional integration event and results in a high efficiency in producing transgenic mice.
    4. Gene integration at the same locus allows a precise comparison of the transgenics from one line to another.

     

    targatt-stemcells-knockin-mouse-2TARGATT Kits

    TARGATT Supporting Materials

     

     

    Mouse Embryonic Fibroblasts (MEF)

    targatt-stemcells-knockin-mouse-3  CF-1
      Neo-resistant
      DR4
      SNL (STO feeder cells)

    SNL (STO feeder cells)

     

    Cell Culture Products

      Germline-tested & ESC-qualified FBS
      Specialty Media
      Basal Media (DMEM)
      Stem Cell Growth and Differentiation Factors
      ASC Small Molecules
      3D Culture and Expansion System

    Reprogramming

    targatt-stemcells-knockin-mouse-4

    targatt-stemcells-knockin-mouse-5

     

     

     

     

     

     

     

    ESC/iPSC Characterization

      Pluripotency Protein Markers                   Stem Cell Gene Array
     targatt-stemcells-knockin-mouse-6    targatt-stemcells-knockin-mouse-7

    ●  Pluripotency mRNA Markers
    ●  Components

     

    ESC/iPSC Differentiation

      Neural Differentiation
      Dendritic Cell (DC) Generation

    ES/iPS Cell Lines

    Mouse ES Cell Lines                                 Human iPS Cells

    targatt-stemcells-knockin-mouse-8   targatt-stemcells-knockin-mouse-9

    Cell Depository

    Primary Cell, cDNA, RNA (Normal)

    targatt-stemcells-knockin-mouse-10  Endocrine
      Neural
      Pulmonary
      Digestive
      Urinary
      Reproductive
      Skeletal Muscle
      Hematopoietic
      Integumentary
      Cardiovascular
      Miscellaneous

    Primary Cell, cDNA, RNA (Disease)

      Blood Disorders
      Neurological Disorders
      Degenerative Disorders
      Metabolic Disorders
      Cardiovascular Disorders
      Congenital Disorders
      Endocrine Disorders
      Autoimmune Disorders
      Genetic Disorders
      Muscular Disorders
      Oncogenic Disorders

    All products - Full view result 

    Read more about Targatt gene modification here

    Published in Promos

    mouseA drug currently approved to treat mouth ulcers has shown promise in animal studies for being a contender in pharmaceutical weight loss. Amlexanox was found by University of Michigan researchers to produce weight loss in obese mice without any change in diet or exercise habits.

    For the study, mice fed a high calorie diet until they became obese were injected with amlexanox. The animals lost weight, despite consuming the same amount of calories. The researchers also noted a loss in overall body fat, a decrease in fatty liver, and a reversal of obesity-induced type 2 diabetes. Once taken off the amlexanox injections, however, the mice experienced weight gain.

    Amlexanox may work by changing the action of genes that control metabolism versus working as an appetite suppressant.

    When the drug was injected in mice, the drug worked by increasing metabolism, not by suppressing appetite.

    "One of the reasons that diets are so ineffective in producing weight loss for some people is that their bodies adjust to the reduced calories by also reducing their metabolism, so that they are 'defending' their body weight," says Dr. Alan Saltiel, the lead researcher at the University of Michigan.

     "Amlexanox seems to tweak the metabolic response to excessive calorie storage in mice."

    The findings were published Sunday in the journal Nature Medicine. Clinical trials are expected to begin later this year to test the drug's effectiveness in humans.

    Published in News
    Monday, 28 January 2013 11:36

    TARGATT Gene Modification

    TARGATT Fast & Efficient Gene Modification in Human Cells. Make your cell line in 3 months!

    Based on our proprietary site-specific TARGATT technology for fast knock-in mouse (KI) generation, Applied StemCell Inc. (ASC) recently developed a TARGATT system specifically for introducing gene(s) of interest into mammalian cell lines. The unique advantage of this system is that any gene of interest can be inserted efficiently into a defined, transcriptionally-active locus with high gene expression in a single copy fashion.

    targat talen knockin celladvantage

    targatt talen knockout genemodification

    Technical Details:

    TARGATT technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses ?C31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by ?C31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.

    pdfMore information - PDF presentation

    knockin knockout targatt talen

    More information about Stem cells

    Prices


    Published in Promos