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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
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    GENTAUR France
    Gentaur Germany

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
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    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    GENTAUR Spain

     GENTAUR Spain
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    Genprice USA
    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
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    GENTAUR Romania

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    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
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    seal-in-search-symantec

     

     

    Wednesday, 19 November 2014 12:23

    OMNIA LH 75 fully automated workstation

    Description:

    OMNIA LH is a fully automated workstation that allows handling of different fluids, dispensing of various components, homogeneous mixing of even small volumes, multiwell plate preparation and replication, biobanking.

    1. easy programming, OMNIA LH can carry out a wide range of protocols accurately and quickly: PCR amplification, real-time qPCR, reverse transcription, methylation, library preparation for next-generation sequencing.

    Genomics

    • DNA-RNA extraction
    • PCR setup
    • Real-time qPCR setup
    • Reverse transcription setup
    • Sequencing setup
    • Agarose gel electrophoresis
    • DNA purification

     

    Sample preparation

    • Plate preparation
    • Plate replication
    • Autosampling
    • Biobanking

     

    Proteomics

    Immunology

    Main features

    • Extreme flexibility of use
    • User-friendly graphic interface
    • Quick programming of every applicable protocol
    • Easy protocol selection from libraries
    • Monitoring of activities, also remotely
    • Sample traceability
    • Customized configuration
    • Modular design
    • Additional devices available

     

    Manufacturer: MASMEC

     

    askus-blue

    Published in Promos
    Friday, 11 October 2013 16:06

    Gene Synthesis

    Gene synthesis is the most cost effective way to enhance your research. In as little as 2 weeks, you can have your Gene cloned in hand and 100% sequence guaranteed. And with Gentaur’s great pricing it can cost less to order a synthetic gene from us than to buy all the kits and reagents necessary to PCR, ligate, clone, grow, purify and sequence your gene of interest. If you like, our free codon optimization will increase protein expression rates and can enhance protein function. In addition our optimization can make previously un-clonable sections of DNA easy to work with. Send us your sequence, Gene ID or an accession number. Let us know what you need and we will deliver!

    Gentaur also offers mutagenesis as well as cloning and subcloning services. Gentaur strives to provide the highest quality synthetic genes at a great price. Our goal is to always provide you with the best value for your research dollar.

    With high throughput DNA synthesis facilities around the world, Gentaur’s daily capacity is unsurpassed. Gentaur is unrivalled in its ability to address the needs of our customers: Whether you need one gene, or one hundred. We respond to your needs–personally.

     

    Features and Benefits

     100% Sequence Guarantee: Individual synthetic genes are confirmed by Sequencing
     Codon Optimization - Free of charge!: Complimentary codon optimization to enhance protein expression and function
     Value Pricing: The best value for your research dollar

     

    Applications

    Antibody Construction
    Antibodies targeted toward specific diseases or targets can be codon optimized for maximum expression in the host organism. Also, an antibody library can be constructed to screen for the most efficient antibody variant.

    Organism Production Optimization 
    Optimize expression of genes related to resource production to maximize industrial biological production efficiency.

    Gene Construction
    Get difficult-to-clone DNA sequences easily and enhance the quality of your research by constructing hypothetical genes.

    Protein Modification
    Codon optimization can increase protein expression efficiency, and a mutant library derived from this process can yield proteins with increased function. Optimizations include secondary structure removal, and repeat reduction as well as organism optimizations.

    Schematics of Gene Synthesis Process

    GeneSynthesisService gentaur

     

    Price:  0.25 euro/base

    Published in Promos

    The polymerase chain reaction (PCR) is a common technique used to amplify, or copy, pieces of DNA. Amplified DNA is then used in genetic analyses for everything from medicine to forensics. In plant research, PCR is a vital step in detecting and sequencing genes, and its applications are endless. However, compounds found in plants often inhibit PCR. Researchers at the University of Southern Mississippi discovered that the use of an additive allows PCR to successfully amplify DNA from once problematic plants.

    PCR is widely used in plant sciences but is not 100 percent reliable. Many plant researchers encounter roadblocks when implementing PCR. For example, many plant species contain phenolic compounds that deter herbivores. These compounds are often extracted along with plant DNA and can stop PCR from working.

    Graduate student Tharangamala Samarakoon and colleagues have found a technique to overcome many of these inhibitory plant compounds. They added a reagent to the PCR mixture that contains three ingredients: trehalose, bovine serum albumin, and polysorbate-20 (all three abbreviated TBT-PAR). "Unlike several other studies, TBT-PAR works at the PCR stage instead of at the DNA extraction stage, so it has promise for pigeon-holed and half-forgotten extractions that previously failed to be amplified using PCR," says Samarakoon. The authors published their research in the January issue of Applications in Plant Sciences.

    Samarakoon tested the TBT-PAR reagent on DNA extracted from tropical and temperate species across four plant families, including Achariaceae, Asteraceae, Lacistemataceae, and Samydaceae. PCR with TBT-PAR successfully amplified DNA for all species, whereas standard DNA extraction and PCR techniques consistently failed.

    TBT-PAR enhanced PCR for DNA extracted from fresh, silica-dried, and herbarium plant material. "Since we study tropical plants, many of which are geographically restricted or rare," explains Samarakoon, "herbarium material is sometimes all that we have available for DNA extraction, and curators are gracious to allow even a small destructive sampling for a single extraction attempt. We want that one attempt, of course, to be successful." Samarakoon predicts that inhibitory plant compounds could be the underlying cause of many PCR failures in herbarium specimens and hopes TBT-PAR will have widespread benefits in herbarium specimen DNA amplification. 

    TBT-PAR was first used in the PCR detection of a shrimp virus by co-author Shiao Wang and his colleagues. "The additive has also been helpful in a colleague's lab where they had trouble amplifying DNA from gopher tortoise ticks, so its utility extends beyond plants," comments Samarakoon. TBT-PAR has the potential for broad use in PCR techniques across DNA samples, species, and taxa.

    The article will be published in the first issue of Applications in Plant Sciences (APPS), a new journal released by the Botanical Society of America. Theresa Culley, Editor-in-Chief of APPS, describes the new journal as a venue to "expedite the dissemination of innovative information encompassing all areas of the plant sciences, including but not limited to genetics, structure, development, evolution, systematics, and ecology."APPS publishes new methods in plant sciences -- an important niche to fill in an age of rapid technological advances.

    Published in News

    Ready-to-use cocktail containing all components,except primer, for the amplification and detection of DNA in real-time quantitative PCR(qPCR).

    The AccuPower® 2X Greenstar qPCR Master Mix is a ready-to-use cocktail containing all components,except primer, for the amplification and detection of DNA in real-time quantitative PCR(qPCR). It combines the automatic "Hotstart" technology of Top DNA polymerase and SYBR Green I fluorescent dye to deliver excellent sensitivity in the quantification of target sequences, with a linear dose response over a wide range of target concentration. Volumes are provided for 100 or 200 amplification reactions of 50ul each.

    accupower-greenstarmaster product f01

    Features and Benefits

    High Specificity : AccuPower® 2X Greenstar qPCR Master Mix provides more accurate Real-time PCR result by application of Hot-start method.
    Stability: The chemical stabilizer maintains enzyme activity for 2 years at -20°C
    Simplicity: Ready to use, AccuPower® 2X Greenstar qPCR Master Mix contains everything of Real-time PCR excluding primer and template.
    Reproducibility: Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment

     

    Applications

    - Real-time quantification of DNA and cDNA targets
    - Gene expression profiling
    - Microbial & Viral pathogen detection

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    Published in Promos
    biotaq
    Applications
     
    • Routine PCR applications
    • TA cloning
    Description

    BIOTAQ™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent.

    BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates (Fig. 1), and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

    BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.

    The specificity and performance of BIOTAQ can be further improved with the use of 2x PolyMate Additive which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

    BIOTAQ™ DNA Polymerase is purified from Thermus aquaticus.
     

    Unit Definition

    One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
    Concentration

    5u/µl
    Storage Conditions

    BIOTAQ can be stored for 12 months at -20°C.

     
    Product Citations
    1. Amaral, I.P. & Johnston, I.A. J. Exp. Biol. 214, 2125-2139 (2011).
    2. Coutinho, C.P., et al. Infect. Immun. 79, 2950-2960 (2011).
    3. Lora, J., et al. PNAS 108, 5461-5465 (2011).
    4. Palazón, A., et al. Can. Res. 71, 801-811 (2011).
    5. del Hoyo, A. & Pedrola-Monfort, Plant Systemat. Evol. 273(3-4), 151-67 (2008).

    Best price on the market: 118 Euro for 500 Units

    PDF-IconDownload datasheet

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    Published in Top Products

    MultiGeneOptiMaxGradientThermalCyclerCommon laboratory research practice utilizes PCR to validate transgenic mouse lines. Validation of these lines typically involve multiple primer sets with various annealing temperatures leading to a very tedious and time consuming process. To allow researchers the opportunity to evaluate multiple transgenes within one PCR reaction, Gentaur offers the MultiGene OptiMax. Traditional thermal cyclers utilize a Peltier microchip block that is enabled for either homogeneous or gradient temperature mode. Additionally, with a traditional thermal cycler, a user can only utilize one annealing temperature per experiment. The new Gentaur MultiGene OptiMax has six distinct Peltier microchip elements that allow users to select up to six different annealing temperatures. This allows for the possibility to evaluate multiple genes in one experiment.

    • We now have a faster machine.
    • Brand new better than gradient function.
    • No more condensation issues
    • Custom block optimisation
    • Brand new PC Viewer

    For more details:

    PDF-Icon Operation Manual

    PDF-IconPresentation

    PDF-IconOptimax app note

    PDF-IconEnduro Catalog ll

    SPECIAL OFFER UNTIL THE END OF 2013

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    Published in Promos

     

    ExiProgen™ is a breakthrough in synthetic biology allowing for the synthesis and purification of one to 16 proteins per run. 
    DNA, in the form of plasmid or linear PCR product, is added to the system and Gentaur’s coupled transcription/translation E. coli extract then synthesizes the protein. 
    The crude protein extract is further purified, using His-Tag engineered into the proteins. 
    The net result is up to 100 ug of >90% pure Protein in about 6 hours. 
    ExiProgen™ can also purify Nucleic Acids from several sources, making it one of the most versatile systems available.

    Features and Benefits
    Built in protocols optimized for protein synthesis/purification and the extraction of a wide variety of nucleic acid samples
    ExiProgen™ has more contains over 900 protocols, each optimized for protein synthesis/purification and target nucleic acid type and source sample. This optimization enables the user to obtain reproducible results for every run, every day. The instrument software can also be upgraded through the network connection port so you can stay up-to-date with the best performing protocols
    Cooling Block
    ExiProgen™ has a built-in cooling block where the elution tube rack sits.
    Sample integrity is ensured by keeping the samples below 10°C.
    This allows for overnight runs and provides you with confidence in your results.
    Magnetic block & Heating block
    To increase extraction efficiency, the ExiProgen™ has an integrated combined magnetic/heating block. The combination of bead magnetization and sample heating reduces experiment time and increases elution efficiency, resulting in increased sample yield. The heating block’s precision temperature control also ensures reproducible results for protein synthesis reactions.
    Contamination Shield
    ExiProgen™ comes with a contamination shield designed to protect the assay from cross-contamination during instrument operation. Any time the pipette tips are moving, the contamination shield will slide under the tips, therefore eliminating the possibility of intra-assay cross-contamination which is a must when working with multiple samples.

    Easy to use LCD Touch screen
    The 3.5" touchscreen maximizes efficiency by offering an intuitive interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.

    ExiProgen f04
    UV lamp
    ExiProgen™ has a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and/or after every nucleic acid extraction or protein purification run. This prevents possible inter-assay cross-contamination that may occur on a busy work day.

    Experimental Procedure

    ExiProgen f05

     

    Principle of protein synthesis and purification
    ExiProgen™ Protein Synthesis Kit useds a E. coli extract to effect coupled transcription/translation of input DNA, which can be plasmid, or PCR generated DNA. The protein itself is generated with a His-Tag, which is then purified using the Ni-NTA magnetic bead provided. The result is high yields of protein that is >90% pure.

    ExiProgen f05 01

    Nucleic acid extraction principle
    ExiProgen™ DNA/ RNA Kits work on the principle of cell lysis, followed by bind, wash elute from silica magnetic beads. High yields of ultrapure DNA or RNA are obtained with OD260 readings of > 1.8 for DNA and 2.0 for RNA.

    ExiProgen f05 02

    And believe it or not it costs ONLY 15700 € !!!

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    Published in Top Products
    Friday, 01 March 2013 13:18

    AccuPower RocketScript Cycle RT PreMix

    AccuPower RocketScript Cycle RT PreMix is a ready-to-use lyophilized mastermix containing all components for first-strand cDNA synthesis from purified Poly(A) or total RNA template. 
    The premix contains Gentaur's exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript, and oligo dT20 for added convenience. Conditions are optimized for Gentaur's patented Cyclic Temperature Reverse Transcription (CTRT) in a premix form.

    Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse 
    transcription of RNA molecules with complex secondary structure. AccuPower RocketScript Cycle RT PreMix has thermostable activity across a wide temperature range, (42°C – 70°C), allowing efficient cDNA synthesis from virtually any RNA. 
    The lyophilized PreMix contains all components necessary for a successful CTRT reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.

    accupower-antibodies-gentaur-aproketscript overview 

    Schematic representation of the 5' UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.

    Features and Benefits

    Thermostable Activity: RocketScript is able to perform reverse transcription reactions throughout a wide range 
    of temperatures from 42°C to 70°C. 

    Enhanced Performance: RocketScript has enhanced performance to handle high and low input RNA concentrations 
    as well as short and long RT target sizes. 

    Easy-to-use: The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse transcription results in the tube. 

    Reproducibility: Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment. 

    Flexible Reaction Conditions: Both CTRT and first-strand cDNA synthesis are both possible, with reaction temperatures 
    ranging from 42°C to 70°C. 

    Convenient: Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided. 

    Stability: Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer 

    RNase, DNase and Proteinase-free: Ensures the integrity of your samples. 

    Broad range of working temperatures: For RNAs that are G:C rich or significant secondary structure 

    Stable for 2 years at -20°C: Long shelf life 

    Specifications

    5' to 3' exonuclease: No
    3' to 5' exonuclease: No
    3’ – A Overhang: No
    Fragment Size: Up to 10 kb


    Application

    - First-strand synthesis of cDNA from RNA molecules (Reverse Transcription)
    - RT-PCR
    - Random priming reaction
    - Library construction
    - Probe labeling
    - mRNA 5’end mapping by primer extension analysis
    - Real time PCR


    Experimental data

    accupower-antibodies-gentaur-aproketscript-cycle figure1

    Figure 1. Comparison of amplification efficiency between AccuPower RocketScript Cycle RT PreMix and competitors’ RTases
    (a) Sensitivity test
    Primer set: Human TFRC set
    Lane M: 1 kb DNA Ladder
    Lane1: 100 ng Human total RNA from HeLa cell
    Lane 2: 10 ng Human total RNA from HeLa cell
    Lane3: 1 ng Human total RNA from HeLa cell
    Lane4: 100 pg Human total RNA from HeLa cell
    RT reaction condition is performed according to each manufacturer’s recommendations

    (b) Full-Length cDNA synthesis test
    RT reactions were performed according to each manufacturer’s recommendation. All cDNAs were amplified with AccuPower Hotstart PCR Premix (K-5050) from Gentaur
    Note: Competitor products show inhibition at high input concentrations of total RNA
    Lane 1: 1 μg Human total RNA from HeLa cell
    Lane 2: 100 ng Human total RNA from HeLa cell
    Lane 3: 10 ng Human total RNA from HeLa cell

    accupower-antibodies-gentaur-aproketscript-cycle figure2

    Figure 2. Complex RNA amplification results of RocketScript Cycle RT PreMix
    Each target gene was amplified after performing reverse transcription with AccuPower RocketScript Cycle RT PreMix.
    Reverse transcription conditions: Conventional 1 hr incubation at 42°C, 50°C, or 60°C, deactivation at 95°C for 5 min
    A: M-MLV Reverse Transcriptase
    B: AccuPower RocketScript Cycle RT PreMix with Oligo (dT)20
    Lane 1: 100 ng Human total RNA from HeLa cell
    Lane 2: 10 ng Human total RNA from HeLa cell

     

    Concentration (copies/rxn) FTRT (Ct) CTRT with 1 cycle (Ct) CTRT with 10 cycles (Ct)
    10,000 19.46 18.77 18.51
    1,000 24.11 24.04 22.93
    100 29.78 28.35 28.19
    10 32.87 33.00 31.05

     

     accupower-antibodies-gentaur-aproketscript-cycle figure3

    Figure 3. Low copy species enrichment by cycle.
    Comparing FTRT (Fixed Temperature Reverse Transcription) to 1 and 10 cycle(s) of CTRT reveal progressive improvement in detected cDNA yield as input copies decrease.

    FTRT: 60 min incubation at 50°C followed by 5 min deactivation at 95°C
    CTRT: Cycles of 37°C annealing 10 sec, 50°C cDNA synthesis 4 min, 55°C secondary structure melting and cDNA synthesis 
    30 sec
    Primer set: Human GAPDH
    Human Total RNA from HeLa cells
    qPCR with AccuPower GreenStar qPCR PreMix (K-6210)

     accupower-antibodies-gentaur-aproketscript-cycle figure4

    Figure 4. Amplification comparison by cycle 

    FTRT (Fixed Temperature Reverse Transcription) conditions:

     

    Step Temperature Time No. of Cycles
    dT20
    cDNA synthesis 50°C 60 min 1
    Heat inactivation 95°C 5 min 1



    CTRT (Cyclic Temperature Reverse Transcription) conditions:

     

    Step Time Temperature No. of Cycles
    dT20
    Primer annealing 37°C 10~30 sec 1, 5, 10, or 15
    cDNA synthesis 50°C 4 min
    Melting secondary structure & cDNA synthesis 55°C 30 sec
    Heat inactivation 95°C 5 min 1

     

    Primer set: Human GAPDH and myc set
    Lane M: 1 kb DNA Ladder
    Lane1: 10 ng Human Total RNA from HeLa Cell
    Lane 2: 1 ng Human Total RNA from HeLa Cell
    Lane 3: 100 pg Human Total RNA from HeLa Cell
    Lane 4: 10 pg Human Total RNA from HeLa Cell

    accupower-antibodies-gentaur-aproketscript-cycle figure5

    Figure 5. Comparison of amplification results between Gentaur RocketScriptTM Cycle RT PreMix and competitor RTases
    All cDNAs were amplified with AccuPower Hotstart PCR Premix (K-5050) from Gentaur
    Primer set: human Myc498 bp set
    Lane M: 1 kb DNA Ladder
    Lane1: 10 ng Human total RNA from HeLa Cell
    Lane 2: 1 ng Human total RNA from HeLa Cell
    Lane 3: 100 pg Human total RNA from HeLa Cell
    Lane 4: 10 pg Human total RNA from HeLa Cell

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    Published in Promos
    Friday, 01 March 2013 13:06

    AccuPower RocketScript RT PreMix

    AccuPower RocketScript RT PreMix contains Gentaur’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript. Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse transcription of RNA molecules with complex secondary structure. 

    RocketScript has thermostable activity (42°C~70°C), allowing efficient cDNA synthesis from virtually any RNA. The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.

    accupower-gentaur-antibodies-aproketscript overview

     

    Schematic representation of the 5’UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail. 

    Features and Benefits 

    Thermostable Activity: RocketScript is able to perform reverse transcription reactions throughout a wide range 
    of temperatures from 42°C to 70°C. 

    Enhanced Performance: RocketScript has enhanced performance to handle both high and low input RNA 
    concentrations as well as short and long RT target sizes. 

    Easy-to-use: The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse transcription results in the tube. 

    Reproducibility: Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment. 

    Convenient: Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided. 

    Stability: Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer 

    RNase, DNase and Proteinase-free: Ensures the integrity of your samples. 

    Broad range of working temperatures: For RNAs that are G:C rich or significant secondary structure 

    Stable for 2 years at -20°C: Long shelf life 

    Specifications

    5' to 3' exonuclease: No
    3' to 5' exonuclease: No
    3’ – A Overhang: No
    Fragment Size: Up to 10 kb


    Application

    - First-strand synthesis of cDNA from RNA molecules (RT)
    - RT-PCR
    - Random priming reaction
    - Library construction
    - Probe labeling
    - mRNA 5end Mapping by Primer Extension Analysis
    - Real time PCR

    accupower-gentaur-antibodies-aproketscript figure1

    Figure 1. Sensitivity comparison between AccuPower RocketScript RT PreMix and M-MLV RTase
    Sensitivity results of AccuPower RocketScript RT PreMix using GAPDH compared with conventional Reverse transcriptases. 
    Each 100 ng – 10 fg of total RNA used for RT and the same amount of RT products used for electrophoresis.
    Lane 1: 10 fg Human total RNA from HeLa cell           Lane 2: 100 fg Human total RNA from HeLa cell
    Lane 3: 1 pg Human total RNA from HeLa cell            Lane 4: 10 pg Human total RNA from HeLa cell
    Lane 5: 100 pg Human total RNA from HeLa cell        Lane 6: 1 ng Human total RNA from HeLa cell
    Lane 7: 10 ng Human total RNA from HeLa cell          Lane 8: 100 ng Human total RNA from HeLa cell

    accupower-gentaur-antibodies-aproketscript figure2

    Figure 2. Comparison of amplification efficiency between AccuPower RocketScript RT PreMix and competitorsM-MLV RTase.
    RocketScript is able to handle a wide range of sample concentrations and transcript lengths so your downstream applications are minimally effected by the reverse transcription step.
    Lanes 1-3: 1,000 ng, 100 ng and 10 ng of total RNA from HeLa cells, respectively.

    accupower-gentaur-antibodies-aproketscript figure3

    Figure 3. Sensitivity comparison between AccuPower RocketScript RT PreMix and competitor RTases using Real Time PCR
    Reverse transcription conditions: conventional 1 hr incubation at 60°C, deactivation at 95°C for 5 min All cDNAs were amplified with AccuPower DualStar™ qPCR PreMix (K-6110) from Gentaur.

    Concentration RocketScript RT PreMix Supplier Q Supplier I
    10,000 23.91 25.63 24.43
    1,000 27.33 28.92 28.03
    100 30.62 32.42 30.88
    10 33.63 35.43 33.95
    Efficiency 104% 103% 108%
    Linearity 0.99999 0.9996 0.9995

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    Published in Promos
    Friday, 01 March 2013 11:48

    AccuPower RT PreMix

    The AccuPower RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix ofM-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal concentrations. Simply add template RNA, primers and water to start your reaction.

    For cDNA amplification with Cyclic RT, please see our AccuPower CycleScript RT PreMix.

     

    Features and Benefits

    Convenient lyophilized RT: Easy to use, simply add your purified RNA
    High yield of cDNA: For genes up to 9 kb within 10 minutes
    Stable for 2 years at -20°C: Long life
    RNase, DNase and Proteinase-free: Ensures the integrity of your samples

     


    Specifications

    5' to 3' exonuclease: No
    3' to 5' exonuclease: No
    3’ – A Overhang: No
    Fragment size: 9 kb


    Application

    cDNA synthesis

    13elisa pcr gentaur taq PyroHotStart HotStart

    Figure 1. Reliability and reproducibility test with AccuPower RT PreMix. Each template showcases amplified target genes.

     

    Lane M: 100 bp DNA Ladder (D-1030)
    Lane 1-4: Reliability test of each lot with AccuPower RT PreMix

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    Published in Promos
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