Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
This email address is being protected from spambots. You need JavaScript enabled to view it.  

Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR France
    Gentaur Germany

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
    Fax (+49) 0241 56 00 47 88 
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it.

     

    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Genprice USA
    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

    seal-in-search-symantec

     

     

    Monday, 16 September 2013 12:22

    Ethidium Bromide Dropper Bottle

    E-Bromide-Dropper-gene-targeting-rosa26-feeder-cells-human-primary-cells-mef cells-ratEthidium Bromide Solution (0.44 mg/ml) is provided in a convenient dropper bottle. A final working cencentration of 0.5 µg/ml is easily obtained by adding 1 drop of solution to 40 ml of staining solution, running buffer or agarose gel.

    Quantity: 5ml, 10ml

    Catalog № 40540004-1

    Price: 74 Euro

    for 5ml 

    Order Button1

    Published in Top Products
    Wednesday, 26 June 2013 15:48

    GelRed Nucleic Acid Gel Stain In Water

    Gelred GelgreenGelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed without changing your existing imaging system.
    GelRed™ can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning.
    A series of safety tests have confirmed that GelRedTM is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal.
    We offer GelRed™ 10,000X solution in DMSO and in water (cat#41003) for better safety. For your convenience, we also offer ready-to-use GelRedTM 3X in water (cat#41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10mL (cat#41003-1).

    FEATURES:
    Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
    Passed environmental safety tests for direct disposal down the drain or in regular trash
    Much more sensitive than EtBr and SYBR Safe
    Available in water, stable at room temperature for long-term storage and microwavable
    - Safer than EB
    - Easy disposal
    - Ultra-sensitive
    - Extremely stable

    Very simple procedures for either precast and post gel staining
    GelRed replaces EtBr with no optical setting change; GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 3)
    Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.
    Simple to use
    Perfectly compatible with a standard UV transilluminator
    Perfectly compatible with downstream applications

    gelred3gelred4Figure 1.GelRed™ is significantly more sensitive than ethidium bromide (EB) for detecting low-level DNA, especially in the lower molecular weight area. Shown left are two-fold serial dilutions of 1 Kb Plus DNA Ladder from Invitrogen electrophoresed on 1% agarose gels precasted with GelRed or EB in 1x TBE. The total amount of DNA loaded per lane was: 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300-nm transillumination and photographed with an EB filter and Polaroid 667 black-and-white print films.

     

     

    The Most Sensitive and Stable Precast Gel Stain

     

    gelred f2

    Figure 2. GelRed™ displays consistently superior sensitivity for post gel staining, regardless of the filter used (A vs. C) and storage and handling condition. SYBR Gold, however, showed comparable performance only when used fresh from the manufacturer and with a SYBR filter (B vs. D). Following a few freeze-thaw cycles, SYBR Gold 10,000X solution degraded significantly, resulting in poor staining (E). SYBR Gold 1X solution also degrades over time (see Figure 4). Two-fold serial dilutions of 1kb Plus DNA Ladder from Invitrogen were electrophoresed on 1% agarose gels in 1x TBE and post- stained with GelRedTMand SYBR Gold, respectively. Gels were imaged using 300-nm transillumination and photographed with the indicated filters and Polaroid black-and-white print films. The total amount of DNA per lane for each serial dilution was: 200 ng, 100 ng, 50 ng and 25 ng from left to right.

    gelred f3

    Figure 3. Excitation and emission spectra of GelRed and GelGreen in the presence of DNA in PBS buffer

    gelred f4

    Note: *GelRed and its uses are covered by pending US and international patents. **SYBR is trademark of Molecular Probes, Inc. and GelStar is trademark of FMC corporation.
    Please also see our EvaGreen™(cat#31000), a breakthrough nucleic acid dye ideally suited for quantitative real-time PCR(qPCR). By incorporating a smart "release-on-demand" DNA-binding technology, EvaGreen™ has low PCR inhibition while exhibiting superior sensitivity. Similar to our GelRed™, EvaGreenTM has remarkable stability.
    Reference:
    For Electrophoretic Mobility Shift Assay: 1. Liu,Y.,et al. Biochemistry, DOI: 10.1021/bi902050p, 2010, 2. Konate, K., et al. Biochemistry, DOI: 10.1021/bi901791x, 2010.

    Catalog number : 41003

    Quantity: 0.5

    Price: 180 Euro

    Order Button1

    Published in Promos