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    Monday, 26 August 2013 17:06

    HIV-1 p24 Antigen ELISA (1x 96 well)

    Virus Core Antigen Detection

    HIV-1 p24 Antigen ELISA 1x 96 well

    - Measures the p24 core protein of HIV-1
    - Quantitation is performed on a standard microplate reader
    - p24 Standard included

    Overview: An anti-HIV p24 monoclonal coating antibody is adsorbed onto a microtiter plate. p24 antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-p24 antibody is added and binds to p24 antigen captured by the first antibody.

    Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-p24. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells.

    A colored product is formed in proportion to the amount of p24 antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HIV-1 p24 protein and sample p24 concentration is then determined.

    Storage: Store all kit reagents at 2° - 8°C. DO NOT FREEZE. When stored properly the kit is stable until the date indicated on the box label.

    PDF-IconDownload datasheet

    Price: 486 Euro

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    3043573-1-400x300

    Catalog number : YK090-SP
    Quantity: 96well

    Product Overview:
    Glucagon is the most important counter-regulatory hormone to insulin in blood glucose homeostasis. It elevates blood glucose levels by stimulating gluconeogenesis and glycogenolysis while inhibiting glycogenesis and gylcolysis in the liver. This hormone is extremely important in studying therapeutic targets for diabetes, particularly Type I.
    Background Information:
    This assay is a chemiluminescent sandwich ELISA and is based on the capture of glucagon molecules in the sample, after extraction and reconstitution. This kit specifically measures intact glucagon, which is unique to many commercially available assays.
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Linearity of Dilution:
    Contact Technical Service for this information
    Sensitivity:
    .003 ng/mL

    Accuracy:
    101%
    Detection Methods:
    Chemiluminescence
    Cross Reactivity:
    Refer to kit protocol for details
    Product Name:
    Glucagon ELISA Kit, Chemiluminescent
    Standard Curve Range:
    0.02 - 2 ng/mL
    Specificity:
    Glucagon (Human, Mouse, Rat and Porcine) 100%
    Oxyntomodulin (Human, Mouse and Rat) <5%
    Glucagon (1-18) 0%
    Glucagon 19-29 (mini Glucagon) 0%
    Analytes Available:
    Glucagon

     Price: 741 EUR

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    Published in Top Products