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GENTAUR Europe

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Gentaur Bulgaria

 GENTAUR BULGARIA
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    GENTAUR France
    Gentaur Germany

      GmbH Marienbongard 20
    52062 Aachen Deutschland
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    Gentaur London

     GENTAUR Ltd. 
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    Gentaur Serbia

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    Other countries

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    seal-in-search-symantec

     

     

    3043573-1-400x300

    Catalog number : YK090-SP
    Quantity: 96well

    Product Overview:
    Glucagon is the most important counter-regulatory hormone to insulin in blood glucose homeostasis. It elevates blood glucose levels by stimulating gluconeogenesis and glycogenolysis while inhibiting glycogenesis and gylcolysis in the liver. This hormone is extremely important in studying therapeutic targets for diabetes, particularly Type I.
    Background Information:
    This assay is a chemiluminescent sandwich ELISA and is based on the capture of glucagon molecules in the sample, after extraction and reconstitution. This kit specifically measures intact glucagon, which is unique to many commercially available assays.
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Linearity of Dilution:
    Contact Technical Service for this information
    Sensitivity:
    .003 ng/mL

    Accuracy:
    101%
    Detection Methods:
    Chemiluminescence
    Cross Reactivity:
    Refer to kit protocol for details
    Product Name:
    Glucagon ELISA Kit, Chemiluminescent
    Standard Curve Range:
    0.02 - 2 ng/mL
    Specificity:
    Glucagon (Human, Mouse, Rat and Porcine) 100%
    Oxyntomodulin (Human, Mouse and Rat) <5%
    Glucagon (1-18) 0%
    Glucagon 19-29 (mini Glucagon) 0%
    Analytes Available:
    Glucagon

     Price: 741 EUR

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    Wednesday, 22 May 2013 16:52

    SafeView™ Nucleic Acid Stain - 1 ml

    photo 0020986SafeView™ products represent a new and safe class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels; a great alternative to more expensive SYBR® Safe or toxic Ethidium Bromide from other competitors. The dyes are developed to replace toxic Ethidium Bromide (EB, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. SafeView™ products are non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

     

    PDF-IconDownload Datasheet

    Price: 66 Euro

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    Friday, 17 May 2013 16:14

    Human Tenascin C Elisa Kit (TNC)

    P63316

    Antigen: Tenascin C (TNC)

    Synonyms: TN, HXB, MGC167029, Hxb, Ten, TN-C, AI528729, MGC144208, MGC144209, cytotactin, tenascin-C, C130033P17Rik, tenc, wu:fk04d02, TNC, tn, MGC140517

    Quantity: 96 Tests/kit

    Components:

    1. Assay plate (12 x 8 coated Microwells). Quantity: 1(96 wells)
    2. Standard (Freeze dried). Quantity: 2
    3. Biotin-antibody (100 x concentrate) Quantity: 1 x 120 µl
    3.HRP-avidin (100 x concentrate). Quantity: 1 x 120 µl
    4. Biotin-antibody Diluent. Quantity: 1 x 10 ml
    5. HRP-avidin Diluent. Quantity: 1 x 10 ml
    6. Sample Diluent. Quantity: 1 x 20 ml
    7. Wash Buffer (25 x concentrate). Quantity: 1 x 20 ml
    8. TMB Substrate. Quantity: 1 x 10 ml
    9. Stop Solution. Quantity: 1 x 10 ml
    10. Adhesive Strip (For 96 wells). Quantity: 4
    11. Instruction manual

    Description Synonyms: CMD1Z, TNC, TNNC, cardiac troponin C|slow twitch skeletal/cardiac muscle troponin C|troponin C, slow|troponin C1, slow

    Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

    Minimum Detection Limit: 0.195 ng/mL

    Detection Range: 0.78 ng/mL - 50 ng/mL

    Assay Precision: 
    Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

    Price: 1087 EUR

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    Thursday, 16 May 2013 14:42

    CA-19-9 ELISA kit

    Name

    CA-19-9 ELISA kit

    Category Name Cancer ELISA kits
    Test 96
    Method ELISA method: Enzyme Linked Immunosorbent Assay
    Principle ELISA principle- Peroxidase conjugated
    Detection Range 0-240U/mL
    Sample 50µl serum
    Specificity 97%
    Sensitivity 5U/mL
    Total Time ~140 min
    Shelf Life 12-14 months

     

    CA-19-9 (Gastrointestinal Cancer Antigen) ELISA Kit which is an enzyme-linked immunosorbent assay for the quantitative measurement of Gastrointensinal Cancer Antigen (CA-19-9) concentration in human serum. An elevated serum CA19-9 suggests the need for further clinical management. The CA-19-9 ELISA kit, like many ELISA KITS, is for diagnostic laboratory use only.

    As in other Cancer ELISA Kits, the CA-19-9 ELISA kit is a solid phase two-site immunoassay. The CA-19-9 ELISA kit is intended to be used as a monitoring and screening test, and is useful for patients in clinical remission, as post-operative serum CA-19-9 values which fail to return to normal, strongly suggest the presence of residual tumor. Tumor recurrence is often accompanied by a rise of serum levels before progressive disease is clinically evident. The sensitivity of the CA-19-9 ELISA kit is 5 U/ml and the specificity is 97%.

    Notable features of Cancer ELISA Kits:

    ▪ User-friendly directions and explanation of test procedures
    ▪ Simple and safe reagent preparation
    ▪ Clear instructions on specimen collection
    ▪ Comprehensive package of required materials
    ▪ Explicit quality control and storage guidelines
    ▪ Reliable and easy-to-read test results

    Product inserts for most Cancer ELISA kits follow a similar method. See the CA-19-9 ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.

    Gentaur also provides other ELISA Kits

    For more information download PDF file 

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    biotaq
    Applications
     
    • Routine PCR applications
    • TA cloning
    Description

    BIOTAQ™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent.

    BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates (Fig. 1), and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

    BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.

    The specificity and performance of BIOTAQ can be further improved with the use of 2x PolyMate Additive which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

    BIOTAQ™ DNA Polymerase is purified from Thermus aquaticus.
     

    Unit Definition

    One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
    Concentration

    5u/µl
    Storage Conditions

    BIOTAQ can be stored for 12 months at -20°C.

     
    Product Citations
    1. Amaral, I.P. & Johnston, I.A. J. Exp. Biol. 214, 2125-2139 (2011).
    2. Coutinho, C.P., et al. Infect. Immun. 79, 2950-2960 (2011).
    3. Lora, J., et al. PNAS 108, 5461-5465 (2011).
    4. Palazón, A., et al. Can. Res. 71, 801-811 (2011).
    5. del Hoyo, A. & Pedrola-Monfort, Plant Systemat. Evol. 273(3-4), 151-67 (2008).

    Best price on the market: 118 Euro for 500 Units

    PDF-IconDownload datasheet

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    Wednesday, 27 March 2013 17:10

    Hybridoma Max Growth Suppliment

    Hybridoma Max Growth Suppliment-280

    Product name: HybridomaMax Growth Suppliment 
    Catalog number: TX-HYB-02
    Quantity: 11ml
    Availability: Yes

    The HybridoMax Hybridoma Growth Supplement replaces feeder layers or other conditioned media and growth factors used in hybridoma production. HybridoMax increases the yield of hybridomas surviving HAT selection, the cloning efficiency of B-cell hybridomas, and the number of antibody producing colonies.

    The HybridoMax Hybridoma Growth Supplement is ready to use. Prepare the serum free Hybridoma growth medium by dissolving 1 bottle of HybridoMax (11 ml) into 1 Liter of D-MEM F-12 (Dulbecco's Modified Eagle's Medium F-12).

    The prepared Serum-Free Hybridoma growth medium is a chemically defined culture medium supporting the serum-free growth of various hybridomas and 293 cells. The compounds of Hybridoma growth medium include water for injection, a modified DMEM/F12 base, HEPES buffer, known amounts of insulin, transferin, testosterone, sodium selenite, ethanolamine, a variety of saturated and unsaturated fatty acids, and stabilizing proteins. HybridoMax contains no IL-6 and no bovine serum albumine or other undefined protein mixtures.

    Price: 137 €

    HYB-05: 5 bottles for 645€
    HYB-10: 10 bottles for 1150€

    PDF-Icon Click here for Datasheet

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    ExiProgen™ is a breakthrough in synthetic biology allowing for the synthesis and purification of one to 16 proteins per run. 
    DNA, in the form of plasmid or linear PCR product, is added to the system and Gentaur’s coupled transcription/translation E. coli extract then synthesizes the protein. 
    The crude protein extract is further purified, using His-Tag engineered into the proteins. 
    The net result is up to 100 ug of >90% pure Protein in about 6 hours. 
    ExiProgen™ can also purify Nucleic Acids from several sources, making it one of the most versatile systems available.

    Features and Benefits
    Built in protocols optimized for protein synthesis/purification and the extraction of a wide variety of nucleic acid samples
    ExiProgen™ has more contains over 900 protocols, each optimized for protein synthesis/purification and target nucleic acid type and source sample. This optimization enables the user to obtain reproducible results for every run, every day. The instrument software can also be upgraded through the network connection port so you can stay up-to-date with the best performing protocols
    Cooling Block
    ExiProgen™ has a built-in cooling block where the elution tube rack sits.
    Sample integrity is ensured by keeping the samples below 10°C.
    This allows for overnight runs and provides you with confidence in your results.
    Magnetic block & Heating block
    To increase extraction efficiency, the ExiProgen™ has an integrated combined magnetic/heating block. The combination of bead magnetization and sample heating reduces experiment time and increases elution efficiency, resulting in increased sample yield. The heating block’s precision temperature control also ensures reproducible results for protein synthesis reactions.
    Contamination Shield
    ExiProgen™ comes with a contamination shield designed to protect the assay from cross-contamination during instrument operation. Any time the pipette tips are moving, the contamination shield will slide under the tips, therefore eliminating the possibility of intra-assay cross-contamination which is a must when working with multiple samples.

    Easy to use LCD Touch screen
    The 3.5" touchscreen maximizes efficiency by offering an intuitive interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.

    ExiProgen f04
    UV lamp
    ExiProgen™ has a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and/or after every nucleic acid extraction or protein purification run. This prevents possible inter-assay cross-contamination that may occur on a busy work day.

    Experimental Procedure

    ExiProgen f05

     

    Principle of protein synthesis and purification
    ExiProgen™ Protein Synthesis Kit useds a E. coli extract to effect coupled transcription/translation of input DNA, which can be plasmid, or PCR generated DNA. The protein itself is generated with a His-Tag, which is then purified using the Ni-NTA magnetic bead provided. The result is high yields of protein that is >90% pure.

    ExiProgen f05 01

    Nucleic acid extraction principle
    ExiProgen™ DNA/ RNA Kits work on the principle of cell lysis, followed by bind, wash elute from silica magnetic beads. High yields of ultrapure DNA or RNA are obtained with OD260 readings of > 1.8 for DNA and 2.0 for RNA.

    ExiProgen f05 02

    And believe it or not it costs ONLY 15700 € !!!

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    Thursday, 14 February 2013 17:20

    NEPTUNE Barrier Tips 10 ul XL Low Retentio

    neptune-10ul Extended Length Barrier Tip Filtered Sterile 10

    Quantity: 4800 (in case - Racked - 96)
    Availability: Yes
    Details:
    Barrier Tips to Give You Complete Peace of Mind
    Protect your work from contamination with one of the industry’s most efficient aerosol filter assemblies. Our proven range of barrier tips reliably prevent aerosol transfer to give you complete peace of mind when performing your most sensitive assays.

     

    bt10xl-series

    BT10XL Series - 10 µl Extended Length Barrier Tip
    GENTAUR offers a complete selection of high quality pipet tips for your entire laboratory's needs.

    Specifications:
    * GLP Compliance Certified
    * S³ Low retention Polymer Technology
    * No detectable nucleic acid
    * No detectable endotoxin
    * No detectable endonucleases
    * No detectable PCR inhibitors
    * Pre-sterilized
    * NEPTUNE S³ Tip without any residue
    * Non S³ Tip with sample residue
    S3 Low Retention Polymer Technology for Total Sample Recovery.
    Exclusively form CLP!
    The trend in research toward ever smaller reaction volumes has many benefits, including improved reaction kinetics, more consistent results and lower overall costs. However, low volume techniques require dispensing very small volumes of reagents with high degree of accuracy and consistency
    Pipet tips produced from standard polymers will variably retain biological solutions, preventing accurate and repeatable results. Diamond polishing of the mold reduces the number of imperfections producing a smoother surface. Silicone treatment of tips further reduces retention, but can leach out and interfere with reactions, or degrade at autoclaving temperatures.
    CLP was the first company to address this challenge and develop a novel polymer technology that produces a Super Slick Surface S3 on plastics. Traditionally, small occlusions and cavities within the molded plastic resulted in significant sample loss. Our third generation S3 polymer system results in a microscopically uniform surface, which virtually eliminates sample hold-up providing the most accurate sample delivery possible. CLP’s S3 polymer combined with our advanced production process produces the most consistent low retention plastics in the industry.

     

    Prices:

    1 box 10x96 Tips for 59 EUR
    10boxes 10x96 Tips + 1 labpet of choice for 480 EUR
    20boxes 10x96 Tips+ 3 labpets for 880 EUR
    30boxes 10x96Tips + 5 labpets for 1290 EUR

    *1250ul are 8x96 in a box

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    Click here for more Barrier Tips products

    Wednesday, 06 February 2013 15:35

    RunBlue DTT Reducer - 1ml

    Catalog number : NXA32001 
    Availability: Yes 

    Details: All RunBlue Buffers, Reagents and Accessories have been specifically designed and formulated for use with the RunBlue Gels and Apparatus to provide optimum performance and results.  Buffers are made usiong the highest qualtiy raw chemicals and have different compositions to other manufacturers buffers - only use RunBlue formulated buffers with RunBlue Gels.

    RunBlue RAPID Running Buffer is a Reducing, Tris-MOPS buffer system which provides a seperation similar to a conventional MES buffer system and provides excellent separation of low molecular weight proteins.  The RunBlue SDS Running Buffer is a Non-Reducing Tris-Tricine buffer system which provides a separation similar to a conventional MOPS buffer system and provides increased separation of higher molecular weight proteins.  Simply choose which buffer will provide the greatest resolution for your protein(s) of interest - see image below.

    SDS-vs-RAPID-Buffers Plain

    Included with the reagents are RunBlue Prestained Markers.  These protein standards have been conjugaded to dyes to enable visualisation of the marker bands in the tank and prior to staining to allow you to see where your protein has reached (very useful for very small proteins!).  In addition, the RunBluePrestain Markers contain two standards (71 & 28 kDa) that have been conjugated to an orange dye to easily see which marker is which during running.

    RunBlue Prestained Markers also offer a quick and convenient method to confirm transfer efficiency when blotting and to compare molecular weight with identity when labelling the protein band.

     

     Price: 50 EUR

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    Wednesday, 06 February 2013 14:34

    PSA ELISA Kit 96t

    Human-ELISA

    Catalog number : 2125-96T
    Quantity:
    Availability: Yes

    Details: This kit is a solid phase-phase sandwich enzyme linked immuno sorbent assay (ELISA). Samples, including standards of known target protein concentrations and unknowns are pipetted into these wells. During the first incubation, the target protein antigen and a biotinylated monoclonal antibody specific for target protein are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove the entire unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of target protein present in the samples.

     

     Price: 173 EUR

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