The AccuPower DNA Ligation PreMix is a lyophilized master mix containing T4 DNA Ligase, ATP, reaction buffer, and patented stabilizer. This DNA ligation premix is conveniently aliquoted in strip-tubes for reactions; you need only add DNAs to be ligated and water. The reaction will work for DNA ligation for all applications: blunt cloning, sticky end cloning and TA cloning. The premix is stable up to four months at room temperature and for three years at -20°C.
Features and Benefits
| Ready to use premix: | Minimal set up time and handling required |
| Fast: | Only 5 minutes for cohesive-end ligation and 10 minutes for blunt-end ligation at room temperature |
| Stable: | Enzyme activity for up to four months at room temperature and for three years in the freezer |
Application
Cloning into vectors, library construction, TA cloning, linker ligation, and re-circlization of linear DNA
Figure 1. Stability test of AccuPower DNA Ligation PreMix at room temperature.
Lane 1 – 12: Lambda DNA / Hind lll fragment (1 µg)
Lane 12 – 24: Lambda DNA / EcoR V fragment (1 µg)
Lane 2, 3, 14, 15: Ligation with AccuPower Ligation PreMix stored at room temperature for 1 month
Lane 5, 6, 17, 18: Ligation with AccuPower Ligation PreMix stored at room temperature for 2 months
Lane 8, 9, 20, 21: Ligation with AccuPower Ligation PreMix stored at room temperature for 3 months
Lane 11, 12, 23, 24: Ligation with AccuPower Ligation PreMix stored at room temperature for 4 months
Figure 2. DNA Ligation efficiency comparison between AccuPower DNA Ligation PreMix and other competitors’ products.
Lane 1, 9: Intact Lambda DNA (1 µg)
Lane 2 – 8: Lambda DNA / Hind lll fragment (1 µg)
Lane 10 – 16: Lambda DNA / EcoR V fragment (1 µg)
Lane 3, 4, 11, 12: Ligation with AccuPower Ligation PreMix
Lane 5, 13: Ligation with T4 DNA Ligase from company N
Lane 6, 14: Ligation with Quick Ligation Kit from company N
Lane 7, 15: Ligation with LigFast Rapid DNA Ligation System from company P
Lane 8, 16: Ligation with Ready-To-Go T4 DNA Ligase from company A
