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    Friday, 01 March 2013 13:45

    AccuPower DNA Ligation PreMix

    Rate this item
    (1 Vote)

    18AccuPowerDNALigationantibodies PreMix DualStar qPCR

    The AccuPower DNA Ligation PreMix is a lyophilized master mix containing T4 DNA Ligase, ATP, reaction buffer, and patented stabilizer. This DNA ligation premix is conveniently aliquoted in strip-tubes for reactions; you need only add DNAs to be ligated and water. The reaction will work for DNA ligation for all applications: blunt cloning, sticky end cloning and TA cloning. The premix is stable up to four months at room temperature and for three years at -20°C.

     

    Features and Benefits

    Ready to use premix: Minimal set up time and handling required
    Fast: Only 5 minutes for cohesive-end ligation and 10 minutes for blunt-end ligation at room temperature
    Stable: Enzyme activity for up to four months at room temperature and for three years in the freezer



    Application

    Cloning into vectors, library construction, TA cloning, linker ligation, and re-circlization of linear DNA

    19taq PyroHotStart HotStart

    Figure 1. Stability test of AccuPower DNA Ligation PreMix at room temperature.

     

    Lane 1 – 12: Lambda DNA / Hind lll fragment (1 µg)
    Lane 12 – 24: Lambda DNA / EcoR V fragment (1 µg)
    Lane 2, 3, 14, 15: Ligation with AccuPower Ligation PreMix stored at room temperature for 1 month
    Lane 5, 6, 17, 18: Ligation with AccuPower Ligation PreMix stored at room temperature for 2 months
    Lane 8, 9, 20, 21: Ligation with AccuPower Ligation PreMix stored at room temperature for 3 months
    Lane 11, 12, 23, 24: Ligation with AccuPower Ligation PreMix stored at room temperature for 4 months

    20pcr gentaur taq PyroHotStart HotStart

    Figure 2. DNA Ligation efficiency comparison between AccuPower DNA Ligation PreMix and other competitors’ products.

     

    Lane 1, 9: Intact Lambda DNA (1 µg)
    Lane 2 – 8: Lambda DNA / Hind lll fragment (1 µg)
    Lane 10 – 16: Lambda DNA / EcoR V fragment (1 µg)
    Lane 3, 4, 11, 12: Ligation with AccuPower Ligation PreMix
    Lane 5, 13: Ligation with T4 DNA Ligase from company N
    Lane 6, 14: Ligation with Quick Ligation Kit from company N
    Lane 7, 15: Ligation with LigFast Rapid DNA Ligation System from company P
    Lane 8, 16: Ligation with Ready-To-Go T4 DNA Ligase from company A

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