Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
This email address is being protected from spambots. You need JavaScript enabled to view it.  

Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR France
    Gentaur Germany

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
    Fax (+49) 0241 56 00 47 88 
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it.

     

    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Genprice USA
    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

    seal-in-search-symantec

     

     

    Friday, 01 March 2013 10:17

    AccuPower Taq PCR PreMix

    Rate this item
    (1 Vote)

    The AccuPower Taq PCR PreMix is a convenient lyophilized PCR master mix containing Taq DNA polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer and is aliquoted in 8-strip PCR tubes. 
    The premix retains its activity for over a month at room temperature and is stable for two years in -20°C freezer. 
    AccuPower Taq PCR PreMix is available with or without tracking dye, depending on your application. If purchased with tracking dye, reactions can be loaded on agarose gels without adding loading buffer.

     

    Features and Benefits

    Ease of use: Just add template and primers and start your reaction. All other reaction components including dNTPs, 
    buffer and enzyme are provided.
    Robust Stability: Stable at room temperature for a month, at 4°C for one year and for 2 years in a -20°C freezer.
    Versatility: The premix is ideal for a wide range of PCR applications.
    Gel loading: Available with or without a tracking dye. Samples with tracking dye can be loaded directly into gels after PCR
    Reproducibility: Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment.

     

    Specifications

    Source: Thermus aquatics
    5' to 3' exonuclease activity: Yes
    3' to 5' exonuclease activity: No
    3' – A Overhang: Yes
    Fragment size: Up to 8 kb from human genomic DNA and 10 kb from Lambda DNA


    Applications

    - Routine PCR
    - Primer extension
    - TA cloning
    - Gene sequencing

    accupower-TaqPCR-gentaur-antibodies-cell-culture-pcr

    Figure 1. Comparison of PCR amplification efficiency between AccuPower Taq PCR PreMix from Gentaur and other suppliers' PCR master mix.

    The cycling conditions for AccuPower Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
    Target Gene : Human myc.
    Lane M : 100 bp DNA Ladder (Gentaur, Cat. No. D-1030)
    Lane 1 : 10 ng of human total cDNA
    Lane 2 : 1 ng of human total cDNA
    Lane 3 : 100 pg of human total cDNA
    Lane 4 : 10 pg of human total cDNA

    accupower-TaqPCR-gentaur-antibodies-cell-culture-pcr-2

    Figure 2. Comparison of PCR amplification efficiency between AccuPower Taq PCR PreMix from Gentaur and other suppliers' PCR master mix.

    The cycling conditions for AccuPower Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
    Target Gene: IRGC (Immunity-related GTPase family, cinema)
    Lane M : 100 bp DNA Ladder (Gentaur, Cat. No. D-1030)
    Lane 1 : 10 ng human genomic DNA
    Lane 2 : 1 ng human genomic DNA
    Lane 3 : 100 pg human genomic DNA
    Lane 4 : 10 pg human genomic DNA

    accupower-TaqPCR-gentaur-antibodies-cell-culture-pcr-3

    Figure 3. Comparison of PCR amplification of long targets between AccuPower Taq PCR PreMix from Gentaur and other suppliers' PCR master mix.

    The cycling conditions for AccuPower Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec, and 68°C for 8 min. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
    Lane M : 1 kb DNA Ladder (Gentaur, Cat. No. D-1040)
    Lane 1 : 3 kb fragment (human tumor protein p53 gene) 
    Lane 2 : 4 kb fragment (human beta globin region)
    Lane 3 : 4.5 kb fragment (human DNA cross-link repair 1A gene)
    Lane 4 : 8 kb fragment (human hemoglobin epsilon 1 gene)

    Order Button1 

    Read 822 times