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    Tuesday, 12 November 2013 10:08

    SensiFAST™ cDNA Synthesis Kit

    Rate this item
    (1 Vote)

    Product description
    This kit is the best option for customers wanting to generate cDNA ready for real-time applications. The Kit provides a rapid and very sensitive method for first strand cDNA synthesis for use in real -time PCR studies. The TransAmp™ Buffer provides highly optimized components for efficient reverse transcription and includes a unique blend of anchored oligo dT and random hexamer primers to ensure unbiased 3’ and 5’ coverage for enhanced data accuracy. An extremely efficient reverse transcriptase delivers highly robust first strand synthesis and higher cDNA yields from a wide range of input RNA concentrations. SensiFAST cDNA Synthesis Kit offers enhanced reproducibility, sensitivity and efficiency for exceptional performance in subsequent real-time PCR experiments.

    Feature: Benefit: Advantege:
    Reproducible Unique reverse transcriptase and new
    TransAmp™ buffer generate
    consistent first-strand cDNA
    Reduces the number of replicates the
    customer needs to do, saving space and cost
    Broad dynamic
    range
    Ideal for dilute and low-copy samples Improves assay sensitivity as abundant and rare transcripts can be run against each other
    Sensitive Real-Time RT-PCR analysis from as
    little as 4pg of starting total RNA
    Can be used even when starting material is limited
    Unbiased cDNA
    synthesis
    Complete 5' to 3' RNA sequence
    representation
    Gives more accurate quantification of genes
    Rapid cDNA synthesis and real-time PCR in
    less than 60 minutes
    Allows higher throughput
    Optimized Designed to complement the
    SensiFAST SYBR and Probe kits
    Can be used directly in a real-time assay without further optimization needed

    Positioning
    The SensiFAST cDNA Synthesis Kit has been optimized for real-time reactions, the new TransAmp Buffer contains reverse transcriptase enhancers that reduce complex RNA secondary structure, so that efficient reverse
    transcription of all RNA samples, regardless of the complexity, can be achieved at 42° C, resulting in unparalleled reproducibility between samples.

    Cat. Product Price Order
    BIO-65053 SensiFAST cDNA Synthesis Kit, 50 Reactions 262 € Order
    BIO-65054 SensiFAST cDNA Synthesis Kit, 250 Reactions 980 € Order

     

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