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GENTAUR Europe

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Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
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    GENTAUR France
    Gentaur Germany

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
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     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
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    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
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    Kuiper 1 
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    Piazza Giacomo Matteotti, 6, 24122 Bergamo
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    Monday, 12 August 2013 12:50

    Isolate Exosomes from Culture Media and Urine with ExoQuick-TC

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    Exo-FBS-exosome-exoquick-gentaur-antibodies-depleted-media-bovineExosomes are 60-120 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids, urine and in media from cells in culture. Exosomes contain distinct subsets of microRNAs depending upon the cell type from which they are secreted. Standard growth medium for most cells in culture require fetal bovine serum (FBS) as a growth supplement to DMEM. FBS is derived from bovine (cow) serum and contains a high abundance of cow exosome vesicles. These exosomes can interfere or cause significant background issues when studying the exosomes secreted from your cells of interest in standard culture conditions.

    Exo-FBS supports equivalent growth of many types of cells in culture, is devoid of cow CD63 positive exosomes and does not have any measurable bovine microRNAs. Perform your studies of cellular secreted exosomes in culture without the worry of contaminating cow exosomes in your experiments. No ultracentrifugation required. Available as standard FBS supplement or heat inactivated FBS media supplement (treated at 65°C for 15 minutes before bovine exosome removal).

    • Exosome-sized vesicles removed
    • FBS that has been stripped of CD63 positive cow exosomes
    • No detectable cow microRNAs
    • Same cellular growth rates supported as standard FBS
    • Use the same as standard FBS (add 10% in DMEM or RPMI)

     

    ExoQuick benefits
    • No time-consuming ultracentrifugation 
    • Less expensive than costly Antibodies and beads 
    • No complicated syringes required 
    • Compatible with biofluid from any species 
    • More effective than any other method 
    • Isolate intact exosomes for functional studies 
    • Use as little as 100 µl of serum 
    • Start with 5 or 10 ml Media or Urine and use ExoQuick-TC

    Click here for more information

     

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