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    Friday, 08 February 2013 12:32

    Kronos Dio

    Rate this item
    (5 votes)

    A luminometer for real-time reporter assay with 8 dish incubator and multicolor measurement system

    Kronos dio AB-2550-kronosDioA luminescence monitoring tool to observe gene expressions with bioluminescent markers under long-term culture. Can be used in studies of internal biological clock, circadian rhythms in organisms, gene silencing, drug stimulation, etc. Kronos Dio is equiped with cell culture system within its housing to provide real-time periodical luminescence measurement with multi-color luciferase assay, enabling users to analyse the effects of complex factors influencing gene expression in the cell or the tissue. Internal temperature control (range: 20 to 45 °C) and carbon dioxide circulation allows for convenient cell culture over a long period of time.

     

    Price: 29 500 Euro / 36 000 USD / 27 600 GBP

     

    kronos-dio-gentaur-colours 

    Installation and training

    For the price GENTAUR provides notebook, installation and training (demonstration of basic software and hardware functionality). 6 hours of installation and training is inclusive in the price at no extra cost for USA and Europe. The necessary materials and tools for connecting the device to the Carbone dioxide source at the expense of the customer.

    20141105 033403

    kronos dio installation

    kronos dio installationkronos dio installation kronos dio installation

     

     

     

     

    Download Catalogue

     

    See also: Cellgraph AB-3000B

    Literature

    Customer references:

    Aaron Rowland, Ph.D.
    Assistant Professor of Toxicology
    Department of Chemistry & Biochemistry
    New Mexico State University
    1175 North Horseshoe Drive
    P.O. Box 30001
    Las Cruces, NM 88003-8001


    We are wanting to explore the ability of human breast cell lines to exhibit circadian rhythm.  We are also interested in influence of environmental toxicants on circadian rhythm as well as general gene regulation.  I felt that the Kronos dio would be the proper equipment to perform this task as it has a CO2 hook up and can monitor 3 colors at once.  Therefore, I could transfect the BASIC luciferase construct using one color and the gene of interest using a different luciferase and monitor reporter activity in real time.  
    Observation that Irritant of Environmental toxin on Human’s breast cell lines reaction and changes.

     

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