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    Articles

    Denaturing Gradient Gel Electroporesis

    Complete Denaturing Gradient Electrophoresis System

    denaturing

     

    Product Description

     

    Denaturing Gradient Gel Electrophoresis (DGGE) is an important technique used in the search for mutations and DNA polymorphisms critical in genetic disorders and cancers, and to understand genetic diversity among species. The new omniPAGE VS20WAVE-DGGE is a flexible system that can be fully customised to perform several different mutation detection techniques. The VS20WAVE-DGGE is a versatile single-unit solution for different single-base pair mutation detection methods. The newly designed VS20WAVE-DGGE is a complete system for DNA mutation analysis.

     

    Using the innovative vertical screw-clamp technology of the new VS20-WAVE system, the VS20WAVE-DGGE is fully equipped with temperature control unit, stirrer, and gradient mixer and casting accessories to perform specific mutation analysis applications. A powerful microprocessor-controlled PID temperature control unit enables various mutation detection techniques to be undertaken between ambient temperature and 70C, while the simple fourscrew design of the WAVE insert accelerates set up of denaturing PAGE gels. Accordingly, the new VS20-DGGE can be used to screen single-base pair changes in the following applications:

     

    • Heteroduplex analysis (HA)
    • Parallel Denaturing Gradient Gel Electrophoresis (DGGE)
    • Constant Denaturing Gradient Gel Electrophoresis (CDGE)

     

    The flexibility provided by the modular design of the VS20WAVE-DGGE and its wide range of accessories enables laboratories to switch quickly and easily between different mutation detection techniques, thereby maximising throughput and screening efficiency. A maximum 96-sample throughput allows detection of as many mutations within a couple hours, alleviating many of the bottlenecks associated with screening for DNA mutations.

     

    GM100 Gradient Mixer ensures efficient gradient formation

     

    The GM100 gradient mixer is supplied as standard to ensure efficient gradient formation by mixing and delivering high- and low-density denaturant solutions. A flat-base design and support handle allows the GM100 to be secured to a retort stand, enabling it to be easily mounted on a magnetic stirring plate (e.g. CSL-STIR), while the mixing chamber can accommodate a magnetic stirrer to form a linear gradient. The MU-D01 peristaltic pump is also recommended for delivery of linear and reproducible gradient gels.

     

    Innovative casting and set-up mechanism

     

    The VS20WAVE-DGGE utilises novel vertical screw clamp technology to assemble two vertical gels. This reduces the number of screws required for set up making casting assembly faster, while a built-in inner buffer chamber within the PAGE insert allows set-up to be completed without the inclusion of heavy top tanks or buffer chambers. A dual purpose PAGE insert eliminates the need for plate transfer, and is used with a cam casting base to guarantee efficient leak free casting.

     

    Utilises the same combs and accessories as standard VS20 systems

     

    The VS20WAVE-DGGE can use all of the combs, glass plates and accessories of existing VS20 units, providing full flexibility. Two 1mm 24-sample combs are supplied as standard while optional 48-sample combs allow screening to take place directly from 96-well thermal cycler blocks after PCR amplification.

     

    Variants:

     

     

    DGGE Package

     

    Product Description

     

    Denaturing Gradient Gel Electrophoresis (DGGE) is an important technique used in the search for mutations and DNA polymorphisms critical in genetic disorders and cancers, and to understand genetic diversity among species. The new OmniPAGE VS20WAVE-DGGE is a flexible system that can be fully customised to perform several different mutation detection techniques. The VS20WAVE-DGGE is a versatile single-unit solution for different single-base pair mutation detection methods. The newly designed VS20WAVE-DGGE is a complete system for DNA mutation analysis.

     

    Using the innovative vertical screw-clamp technology of the new VS20-WAVE system, the VS20WAVE-DGGE is fully equipped with temperature control unit, stirrer, and gradient mixer and casting accessories to perform specific mutation analysis applications. A powerful microprocessor-controlled PID temperature control unit enables various mutation detection techniques to be undertaken between ambient temperature and 70C, while the simple fourscrew design of the WAVE insert accelerates set up of denaturing PAGE gels. Accordingly, the new VS20-DGGE can be used to screen single-base pair changes in the following applications:

     

    • Heteroduplex analysis (HA)
    • Parallel Denaturing Gradient Gel Electrophoresis (DGGE)
    • Constant Denaturing Gradient Gel Electrophoresis (CDGE)

     

    The flexibility provided by the modular design of the VS20WAVE-DGGE and its wide range of accessories enables laboratories to switch quickly and easily between different mutation detection techniques, thereby maximising throughput and screening efficiency. A maximum 96-sample throughput allows detection of as many mutations within a couple hours, alleviating many of the bottlenecks associated with screening for DNA mutations.

     

    GM100 Gradient Mixer ensures efficient gradient formation

     

    The GM100 gradient mixer is supplied as standard to ensure efficient gradient formation by mixing and delivering high- and low-density denaturant solutions. A flat-base design and support handle allows the GM100 to be secured to a retort stand, enabling it to be easily mounted on a magnetic stirring plate (e.g. CSL-STIR), while the mixing chamber can accommodate a magnetic stirrer to form a linear gradient. The MU-D01 peristaltic pump is also recommended for delivery of linear and reproducible gradient gels.

     

    Innovative casting and set-up mechanism

     

    The VS20WAVE-DGGE utilises novel vertical screw clamp technology to assemble two vertical gels. This reduces the number of screws required for set up, making casting assembly faster. A built-in inner buffer chamber within the PAGE insert allows set-up to be completed without the inclusion of heavy top tanks or buffer chambers. A dual purpose PAGE insert eliminates the need for plate transfer, and is used with a cam casting base to guarantee efficient leak free casting.

     

    Utilises the same combs and accessories as standard VS20 systems

     

    The VS20WAVE-DGGE can use all of the combs, glass plates and accessories of existing VS20 units, providing full flexibility. Two 1mm 24-sample combs are supplied as standard while optional 48-sample combs allow screening to take place directly from 96-well thermal cycler blocks after PCR amplification.

     

    Variants:

     

     

    Denaturing Gradient Electrophoresis Temperature Control Unit 

    VS20WAVE-DGGETC-600x600

    Product Description

    The redesigned VS20DGGETC temperature control unit combines buffer recirculation with a heat sensor and 1.4kW heating element to facilitate precise temperature control to within 0.02C, allowing the gel temperature to be set to the melting temperature (Tm) of the amplified DNA polymorphism or mutation of interest. Other benefits include; a conspicuous 4-digit 16mm LED panel to aid set-up, precise tuning to within 0.1C resolution, an operating set point, plus three adjustable pre-set temperature values, and stirred buffer circulation for temperature stability and uniformity.

    Programmable power supply option

    At 500V, 800mA and 300W outputs, the optional CS-500V power supply provides full flexibility for different mutation detection techniques.

    Variants:

     

    Vertical Accessories

    Large & Small Glass Plate Racks

    BC06-glass-drying-rack-600x600

     

    Product Description

    These sturdy racks are designed for safe drying and storage of glass plates.

    Variants:

    CSL-MGR – Mini Glass Plate Rack for 20 x 2mm plates

    CSL-LGR – Large Glass Plate Rack for 10 x 5mm plates

     

    Gradient Mixers

    BC gradmix-600x600

    Product Description

    Ideal for Caesium, Sucrose and Gel gradients these Gradient Mixers comprise two chambers a reservoir and a mixing chamber with an interconnecting valve. A second valve regulates the output flow from the mixing chamber. All mixers have a flat base which allows them to be placed on a magnetic stirrer. A magnetic stirring bar can be placed directly in the mixing chamber to ensure a constant gradient. The support rod allows the mixer to be fixed to a retort stand for extra stability.

    Variants

    Multiple Minigel Casting

     

    Multi-casters-600x600

    Product Description

    Advance casting of multiple mini gels can help to achieve consistent results between runs. These multiple gel casting systems are tailored for use with omniPAGE mini vertical electrophoresis units; with three models of 6, 12 and 24 gel capacities. Fewer gels can be poured if required using the acrylic saver blocks supplied with each system.

    The fixed hinged clamps allows the gel sandwich to be adjusted to the correct pressure irrespective of the number or the thickness of gels being poured. Separation sheets allow the easy separation of the glass plates after pouring.

    Variants

    Mini Vertical Units

    The OmniPAGE Mini, Mini Wide, Maxi and the new VS20 WAVE systems all comprise a modular tank design with dedicated inserts for PAGE, blotting and capillary gel isoelectric focusing (IEF).

    OmniPage Mini Vertical Systems

    OmniPAGE mini

    CVS10DSYS-600x600

    Product Description

    Gentaur Mini Vertical systems are predominantly used for protein electrophoresis. They include the OmniPAGE CVS10TETRAD systems equipped with enough combs and glass plates to run 4 gels, and the standard OmniPAGE CVS10 systems, which accommodate up to 2 handcast or commercial precast gels.

    By combining both functionality and ease of use, the OmniPAGE CVS10TETRAD and standard OmniPAGE CVS10 systems set the bench mark for simple, versatile vertical mini gel electrophoresis. Each OmniPAGE electrophoresis system can accommodate up to 4 handcast gels and 2 commercial precast gels to provide complete flexibility for individual research needs, while unique sliding clamp technology within the PAGE insert facilitates fast, intuitive leak-free casting.

    With a trouble-free set up and consistent performance, the OmniPAGE Mini Vertical systems are perfect for today’s laboratories, where the ability to generate reproducible results quickly is essential.

    Variants:

    • CVS10PRE - OmniPAGE Mini, 10 x 10cm Dual. No accessories. CLAMP VERSION

     

    OmniPAGE Mini Wide

    VS10WD-600x600

     

    Product Description

    The OmniPAGE Mini Wide vertical gel unit with an active gel width of 18cm effectively allows double the number of samples to be resolved as the OmniPAGE Mini unit. This allows consistency of sample comparison on a single gel and is designed for those with greater than 20 samples to compare and resolve.

    Simple set up using ultra soft silicone seals guarantees trouble free glass plate loading and gel casting. Dual gaskets on the gel running insert along with notched and plain glass plates ensure leak proof gel running. Rapid set up cooling retains resolution in extended separations and also saves on buffer volume without affecting run quality.

    4mm thick glass plates reduce breakage and have bonded spacers for added convenience. A wide range of accessories is available allowing many techniques to be performed using the same unit. Prep combs can be used to maximise sample loading and recovery. Accessory electroblotting and tube gel modules are available which use the same outer tank and lid.

    Variants:

    OmniPage Maxi Vertical Systems

    VS20WAVE Maxi

    WAVESYS-CU-600x600

     

    Product Description

     

    The new VS20 WAVE Maxi System is Gentaur latest product innovation for large format vertical gel electrophoresis. Designed to perform a variety of separations, including first and second-dimension SDS-PAGE, native, preparative, gradient and high-resolution nucleic acid electrophoresis, plus capillary tube gel IEF and electroblotting, the VS20 WAVE is one of the most versatile maxi vertical systems available.

     

    By introducing innovative, new vertical screw-clamp technology within the PAGE insert only four screws are now necessary to secure as many 20x20cm gels. This gives the VS20 WAVE Maxi the selective advantage of a much faster set up speed compared to competitor products whose traditional clamping configurations require as many as 24 screws to secure just two glass plates. In addition, the WAVEs vertical screw-clamp configuration distributes pressure evenly along the height of the gel rather than in the centre to eliminate plate bowing and gel compression, but still maintains a leak-proof seal during casting; while the ergonomic wave-like design of the PAGE insert aids both handling and set up.

     

    Whatever your requirements are the WAVE can be made to meet them. Regardless of whether it is running 2 or 4 gels, electroblotting, and IEF using capillary tube gels or IPG strips, all of these techniques may be performed using the same omni-purpose unit while retaining the benefits of large format electrophoresis, such as extended separation distances, greater sample throughput and superior resolution.

     

    Variants

     

     

     

     

    OmniPAGE Maxi

    CSL-omniPAGEmaxi-EPcompsCB-600x600

     

    Product Description

     

    The OmniPAGE Maxi, the preferred unit for maxi protein electrophoresis, combines convenient ease of use features with high resolution separations. Simple set up using ultra soft silicone seals guarantees trouble free glass plate loading and gel casting.

     

    Dual gaskets on the gel running insert, along with notched and plain glass plates, ensure leak proof gel running. Rapid set up cooling retains resolution in extended separations and also saves on buffer volume without affecting run quality.

     

    Glass plates reduce breakage and have bonded spacers for added convenience. A wide range of accessories is available allowing many techniques to be performed using the same unit. Prep combs can be used to maximize sample loading and recovery. Accessory electroblotting and tube gel modules are available which use the same outer tank and lid.

     

    Variants:

     

     

     

     

    Complete WAVE Maxi System for 2-D Electrophoresis & High Intensity Blotting

    VS20WAVECES-2-600x600

    Product Description

    The VS20 WAVE complete electrophoresis system provides a fully integrated solution using one universal gel tank and lid for vertical PAGE, tube gel IEF and electroblotting.

    • Interchangeable modular inserts allow users to combine vertical PAGE with capillary gel IEF to perform 2-D electrophoresis, followed by western transfer for enhanced sensitivity
    • Scale-up your discovery projects using a large format, high-resolution system
    • Cooling supplied for temperature-sensitive separations and samples
    • High-intensity blotting system available for rapid transfers

    Package includes:

    • 1x WAVE Maxi Vertical Unit which includes: PAGE insert, 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick 24 samples), 1x casting base, silicone mat, cooling coil
    • 1x WAVE High Intensity Electroblotting Module which includes: WAVE Maxi Plate Electrode Blotting Insert, 1x compression cassette and 6x fibre pads
    • 1x WAVEDCI Capillary Electrophoresis Module which includes: WAVE Maxi Tube Gel Insert, 100x capillary tubes,10x blanking ports
    • 1x WAVE IEF-KIT: 1 set of plain glass plates with bonded spacers, 0.6x20cm (w x h); and 2x 2-D combs with one 3.5mm marker lane and one 18cm preparatory well
    •  
    • Complete WAVE Maxi System for 2-D Electrophoresis & Blotting

    VS20WAVECES-600x60099

    Product Description

    The VS20 WAVE complete electrophoresis system provides a fully integrated solution using one universal gel tank and lid for vertical PAGE, tube gel IEF and electroblotting:

    • Interchangeable modular inserts allow users to combine vertical PAGE with capillary gel IEF to perform 2-D electrophoresis, followed by western transfer for enhanced sensitivity
    • Scale-up your discovery projects using a large format, high-resolution system
    • Cooling supplied for temperature-sensitive separations and samples
    • High-intensity blotting system available for rapid transfers

    Package includes:

    • 1x WAVE Maxi Vertical Unit which includes: PAGE insert, 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick 24 samples), 1x casting base, silicone mat, cooling coil
    • 1x WAVE Standard Electroblotting Module which includes: WAVE Maxi Platinum Wire Blotting Insert, 4x compression cassettes and 18x fibre pads
    • 1x WAVEDCI Capillary Electrophoresis Module which includes: WAVE Maxi Tube Gel Insert, 100x capillary tubes,10x blanking ports
    • 1x WAVE IEF-KIT: 1 set of plain glass plates with bonded spacers, 0.6x20cm (w x h); and 2x 2-D combs with one 3.5mm marker lane and one 18cm preparatory well.

     

    Complete System for Maxi Vertical Electrophoresis

    VS20D-BI-DCI-600x600--

     

    Product Description

    The omniPAGE range of Modular Vertical Gel Systems allow multiple electrophoresis techniques to be performed in the same unit. These systems include all modules and accessories required for Slab Gel Electrophoresis, 2-D Electrophoresis and Electroblotting. The central component is the omniPAGE Mini Vertical unit, Mini Wide Vertical Unit or omniPAGE Maxi Vertical unit. These all include a rapid and intuitive casting system, enhanced and easy to set up cooling system and increased capacity to run up to four gels per run. In addition, the omniPAGE Tube gel module is capable of resolving up to 10 first dimension gels and the Electroblotting module has a four blot capacity for the Mini system, and a three blot capacity for the Mini Wide and Maxi. The package includes all the necessary accessories for Slab Gel, First Dimension and Electroblotting. Each of these techniques benefits from rapid set up cooling packs which provide enhanced resolution even during high intensity 2-D electrophoresis and Electroblotting.

    Package includes:

    • 1x omniPAGE Maxi Vertical Unit which includes: 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick 24 samples), 1x casting base, silicone mat, cooling pack
    • 1x Capillary Electrophoresis Module which includes: internal running module for tube gels, capillary tubes, blanking plugs, 2-D combs and spacer
    • 1x Electroblotting Module comprising: internal electroblotting module, 3x compression cassettes for gel sizes up to 20x20cm and 12x fibre pads.