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    Articles

    Nanoliter injector

    For oocyte injection and applications in the 2 to 70 nanoliter range

    Nanoliter injector

    WPI’s microprocessor-controlled Nanoliter 2000 uses direct piston displacement. By either pushing the injection button on the control box or pressing on the optional footswitch, a calibrated volume will be smoothly injected. The process is quiet and vibration free. Capillary filling and injection speeds are 23 nL/sec and 46 nL/sec (emptying speeds are 92 nL/sec and 230 nL/ sec). Maximum fluid ejection is 5 μL. Each unit comes with sufficient glass to pull at least 300 tips. Glass is 1.14 mm O.D. (nominal) and 0.5-mm I.D. By setting the DIP switch, the injection volume can be changed from 2.3 to 69.0 nL in 16 steps. Up to 100 injections may be triggered per filling. Since the volume of a normal Xenopus oocyte is about 500 nL, the instrument has the capability to inject from less than 1% to over 10% of the total volume of the oocyte in one preset step increment. Included: 1 vial 3.5 in. capillaries (100); 1 vial 7 in. Capillaries (100); replacement “O” rings; Allen wrench; MicroFilTM MF34G backfilling needle; and two sample μTip™ pre-pulled micropipettes.

    Smart Controller: Micro4, an optional microprocessor-based controller, can provide an “intelligent” and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane keypad and LCD display. From the keypad the user can set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, and rate of delivery, as well as synchronize the starting and stopping of a combination of Injectors. User parameters can be stored in the device’s “non-volatile” memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for “hands free” start/stop operation. An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs.

    NANOLITER 2000

    SPECIFICATIONS
    INJECTION VOLUME Variable
    REMOTE CONTROL Yes
    GLASS OD 1.14 mm
    GLASS ID 0.5 mm
    STEP 12.7 μ/step
    INJECTION SPEED
    Slow 23 nL/sec
    Fast 46 nL/sec
    FILL SPEED
    Slow 23 nL/sec
    Fast 46 nL/sec
    EMPTY SPEED 92 nL/sec
    VARIABLE VOLUME RANGE 2.3 - 69.0 nL
    SMALLEST VOLUME 2.3 nL
    TO CHANGE VOLUME Set switch
    INJECTIONS PER FILLING, MAX. 100 injections
    SHIPPING WEIGHT 3 lb. (1.1 kg)