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    Articles

    Programmable two-stage micropipette puller

    Programmable two-stage micropipette puller

    PUL-2 is a microprocessor-controlled two-stage puller for making glass micropipettes or microelectrodes used in intracellular recording, patch clamp studies, or microinjection. It can produce pipettes with tip diameters from less than 0.1 micron to tens of microns. Heat setting is digitally controlled with 0.1% accuracy. Pulling length can be adjusted to control the taper of the pipette. This two-stage puller is a compact, versatile, reliable workhorse at a very reasonable cost. The microprocessor in combination with LED, digital display and sound generator makes the PUL-2 easy to use and foolproof. Controls are intuitive and self-explanatory. Every keypad function is confirmed or rejected with an audible signal. The digital display indicates heat setting or the status of an operation. An LED indicator confirms that the glass capillary is properly installed at the initial pulling position for better reproducibility. Two-stage pulling is automatically accomplished according to the microprocessor setting, with no need of hand intervention. Heat settings for both stages can be stored in memory. The permanent memory stores up to 99 heat settings. Another unique feature of this puller is the high quality switching power supply that not only reduces size and weight and can be used anywhere in the world without worry about the line voltage differences, but also solves the problem of pulling reproducibility due to line voltage fluctuation. Heating voltage can be controlled within 0.1% accuracy even when line voltage fluctuates from 90 to 240 VAC.

    The importance of the multistage puller A single-stage puller is good only for producing intracellular electrodes that have less than 0.1 micron tips. When the pull starts, the glass is too soft for a larger tip to form. For pulling pipettes with a tip larger than 0.1 micron, a two (or more) stage puller is required. The first pulling stage produces a thin section on the capillary. The pulling force is then more concentrated on the thin section, on which the stress is increased during the second pulling stage. With concentrated force during second stage pulling, the capillary can be separated at a lower temperature (i.e., higher glass viscosity) to form a larger tip. By controlling the pulling length of the first pull, PUL-2 can produce tapers suitable for most applications.

    PUL-2 Specifications
    Heater Element Nickel-chromium coil
    Pulling Force Gravity, selectable at
    250g & 330g
    Max. Capillary Length 210 mm
    Min. Capillary Length 55 mm
    Memory 99 sets
    Power 90-240 V, 50/60 Hz.
    Dimensions 21x 21 x 20 cm
    Shipping Weight 15 lb