Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
This email address is being protected from spambots. You need JavaScript enabled to view it.  

Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
    This email address is being protected from spambots. You need JavaScript enabled to view it.
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Germany

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
    Fax (+49) 0241 56 00 47 88 
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it.

     

    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Genprice USA
    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

    seal-in-search-symantec

     

     

    Articles

    EPC 8 Patch Clamp Amplifier

    With his selectable manual or digital control the EPC 8 is the amplifier of choice for every electrophysiological laboratory

    EPC EPC 8 8 Patch Clamp Amplifier Patch Clamp Amplifier

     

    The letters EPC stand for Extracellular Patch Clamp, the method introduced by Neher and Sakmann (1976) for recording the currents in a small patch of membrane under voltage clam technical advances have occurred, most notably the discovery of the 'gigaseal' by E. Neher (1981), which have made the words extracellular and patch excessively restrictive in describing this instrument. Based on the excellent design of the EPC 7 improvements and extensions were made which make the EPC 8 a worthy successor of the EPC 7. In comparison to the EPC 7 the holding potential range is increased to +/- 500 mV and a new low gain range allows to measure currents up to 2 µA. A fast current-clamp mode is introduced, which allows better follow of rapid changes in membrane potential, such as in neuronal action potentials. The EPC 8 can be controlled manually like the EPC 7 by use of the knobs and switches on the front panel, but most of its functionality can also be controlled by use of a computer via appropriate hardware. The EPC 8 is adapted for use with ITC-16 and PULSE, but it can also be used with other data acquisition hardware and software.

    Features of the EPC 8:
    • Manual control by use of the knobsand switches on the front panel
    • Digital control of the following functions by the use of a computer: Gain, Mode of the EPC 8 (Voltage-clamp, Current-clamp etc.), Filter, 'Phase tracking'
    • Parameters which are manually set on the front panel (except 'Phase tracking') can be read by the software.
    • Holding potential range of +/-500 mV +/-200 mV pipette offset
    • A low gain range allows currents up to 2 µA Built-in 'phase tracking' capabilities
    • Variable hardware filters
    • C-Slow compensation (0-1000 pF, calibrated)
    • 100-130 V AC or 200-260 V AC switchable
    • A fast current clamp mode allows better follow of rapid changes in membrane potential, such as in neuronal action potentials
    • Fast current clamp is also possible in the low gain range
    • RS compensation and C-Fast cancellation are active in current clamp mode
    •Only one current monitor output with a variable 7-pole Bessel filter in 12 steps: 0.1, 0.3, 0.5, 0.7, 1, 3, 5, 7, 10, 20, 30 kHz and FULL (Full with a 3-pole Bessel filter)
    • Built-in 'phase tracking‘ capabilities according to Fernandez, by use of 'dithering‘ an additional resistor in series with the bath electrode or an additional capacity in the C-fast circuit.

    Set-up a complete patch clamp data acquisition system with the EPC 8, HEKA's LIH 1600 acquisition interface, and PATCHMASTER, the electrophysiology software.

    The following different hardware configurations of a computer controlled EPC 8 system are possible:

    Besides the computer one of the following hardware is required to control the EPC 8:

    • LIH 1600 with PCI-1600 board
    • ITC-18 with PCI-18 board
    • any other 2 x 16 bit parallel digital I/O board

    Establish software control by using
    • PATCHMASTER, PULSE or TIDA in conjunction with the LIH 1600 or ITC-18 or
    • write your own acquisition software for LIH 1600 or any other 2 x 16 bit parallel digital I/O board.

    The documentation about control of the EPC 8 via LIH 1600 is included with the EPC 8 delivery volume thus allowing the use of this patch clamp amplifier together with your own programmed data acquisition software. So anybody can use this patch clamp amplifier with his own data acquisition software.

    EPC 8 Main Unit
    Dimensions:
    (25.0 x 48.3 x 9.0) cm,
    (9.8 x 19.0 x 3.5) inch
    Weight:
    7.6 kg, mounts in a 19” rack.
    Operates on standard 115 V / 230 V.
    EPC 8 Headstage
    Dimensions:
    (40 x 70 x 19) mm,
    (1.57 x 2.75 x 0.75) inch
    The input circuitry is contained in a hybrid integrated circuit.
    Current measuring resistors:
    50 GΩ (high range)
    500 MΩ (medium range)
    5 MΩ (low range)
    Largest measurable currents:
    200 pA (50 GΩ range)
    20 nA (500 MΩ range)
    2 mA (5 MΩ range)
    Noise measurements with 4-pole
    Butterworth or Bessel filter:
    DC to 1 kHz: <50 fA
    DC to 3 kHz: <100 fA
    DC to 10 kHz: <300 fA
    Input connector:
    Standard BNC
    Other connections:
    Ground sense input, Pipette command output
    Filters
    The current monitor signal of the EPC 8 is filtered by a 7-pole Bessel filter from 0.1 - 30 kHz in 11 steps. In the additional 'FULL‘ setting a 3-pole Bessel filter is used and the current signal is provided at full bandwidth of the EPC 8.

    Capacitance Compensation
    Manual adjustment of the fast and slow capacitance
    cancellation.
    C-fast:
    0to10pF,calibrated
    0.5to5mstimeconstant
    C-slow:
    0.2to10pF,calibrated
    2 to 100 pF, calibrated
    10 up to 1000 pF depending on R-membrane, calibrated
    Series conductance adjustment:
    0.01 to 1 mS, calibrated
    Series Resistance Compensation
    Automatically determined from the capacitance transient
    cancellation controls.
    Fractional compensation:
    0-90%

    Pipette Offset
    Adjustement of the pipette offset in a range of +/-200 mV
    Holding Potential
    Hardware controlled holding potential with a total range of +/-500 mV.
    Noise Monitor Signal
    Gain:
    0.005 to 1000 mV/pA
    Bandwidth:
    100 kHz (medium and low range) - 60 kHz (high range)
    Current Clamp
    Range:
    100 nA (low range) - 1 nA (medium range)
    Mode:
    Fast and slow
    Computer Interface
    Computer controlled operation via 2 x 16 bit parallel output board, LIH
    1600 or ITC-18 possible
    Related Products
    LIH 1600
    16-bit Multi-channel data acquisition system
    PATCHMASTER
    Multi-channel patch clamp data acquisition and analysis software for
    Windows and MacOS.
    PULSE/PULSEFIT
    Patch clamp data acquisition and analysis software for Windows and
    MacOS.
    X-CHART
    Software implemented chart recorder for Windows and MacOS..
    FURA
    Synchronized patch clamp and fluorescence measurements.
    TIDA
    Electrophysiological data acquisition and analysis software for
    Windows.
    EPC 10
    The fully computer controlled patch clamp amplifier with built-in
    interface board.
    PIP 5
    Temperature controlled micro pipette puller.
    MIM 4
    Motorized 3-axis micromanipulator with video option.