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    Articles

    MultiClamp MultiClamp 700B 700B Microelectrode Amplifier Microelectrode Amplifier

    A Computer-Controlled Dual Channel Resistive-Feedback Patch Clamp and High-Speed Current Clamp Amplifier

    MultiClamp MultiClamp 700B 700B Microelectrode Amplifier Microelectrode Amplifier1

    INTERNAL AUTOMATIC MODE SWITCHInstantly switch recording modes by using the membrane potential as the trigger. Pre-set a voltage threshold in current-clamp mode, and MultiClamp 700B will automatically switch to voltage-clamp when the membrane potential reaches that voltage level. User-specified delay will allow you to perform creative new experiments.

    OSCILLATION SUPPRESSIONSudden changes in membrane or pipette parameters may result in undesirable oscillations during whole cell recording. MultiClamp 700B will detect current or voltage oscillations and automatically disable or intelligently reduce compensation settings to protect your cell from damage.

    SLOW CURRENT INJECTIONSlight voltage drift—often due to changing electrode properties—may contaminate an otherwise decent current-clamp recording. In order to maintain the membrane potential at a consistent level, MultiClamp 700B will automatically inject compensatory current over a user-defined time course.

    MultiClamp Software Control

    Instead of the usual front panel knobs and switches, the MultiClamp 700B is controlled by the MultiClamp 700B Commander, a program that runs on a host computer (PC and Macintosh; see Requirements below) and communicates with the amplifier via a USB cable. This control interface provides automation of bridge balance, pipette offset, pipette and whole-cell capacitance compensation, in addition to "smart" features such as protection from oscillations and threshold-based mode change. Amplifier settings such as gain, filter frequency, whole-cell capacitance, recording mode and also input / output scale factors are automatically telegraphed to the data acquisition software via "messaging" through the computer operating system.

    SoftPanel conventional control

    Although the MultiClamp 700B is a computer-controlled amplifier, the mouse and/or keyboard is not the only means of controlling the instrument. The optional SoftPanel was designed for those who prefer a more conventional feel to amplifier control. By way of a USB connection, the SoftPanel physically replicates all essential amplifier functions by acting as a hardware extension of the MultiClamp 700B Commander software. SoftPanel knobs replicate continuous mouse controls ("gliders"), while buttons replicate single-click mouse controls.

    Headstage

    The MultiClamp 700B comes standard with two identical but independent CV-7B headstages, each of which contains both current-to-voltage and voltage-following circuitry. This design allows the user to rapidly switch between patch clamp recording and true high- speed current clamp recording. Thus, with two headstages, the MultiClamp 700B can perform the function of two patch clamps, two current clamps, or a combination of patchand current clamp amplifiers. Furthermore, two optional voltage-follower headstages (HS-2 type) can be connected to auxiliary inputs to allow third and fourth-point voltage recording.

    The CV-7B was designed for low noise and flexible recording features. However, some specialized applications require even more flexibility. For example, the rather large membrane capacitance in bilayer recording demands greater capacitance compensation. The optional CV-7B/BL headstage was developed for this purpose. Another optional headstage, the CV-7B/EC, was designed for large (± 2 V) commands required during electrochemistry recording (amperometry, voltammetry).