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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
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    GENTAUR France
    Gentaur Germany

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
    Fax (+49) 0241 56 00 47 88 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
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    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
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     GENTAUR Spain
    Tel 0911876558
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    Gentaur Serbia

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    GENTAUR Greece

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    Other countries
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    Articles

    General Kits

    BioReady Taq pac.

    BioReady Taq is a highly pure, thermostable recombinant DNA polymerase encoded by a modified gene from Thermus aquaticus species and expressed in E.coli. Its recombinant nature ensures utmost purity, reproducibility and processivity. BioReady Taq processes an initial 5’→3’ exonuclease activity and lacks 3’ →5’exonuclease activity. The enzyme leaves a single 3’-nucleotide overhang that makes the product for cloning by TA vector system. BioReady Taq provides a strong thermostability that meets the requirements of specialized PCR applications.
    This product includes all components except primers. Users only need to prepare primers and template to run PCR. The operation is rapid and simple.
    Features
    Good thermostable
    High activity
    The PCR product with this enzyme is available to run TA clone directly
    The product is suitable for real-time PCR reaction owing to 5’-3’ exonuclease activity.

    Application
    PCR raction
    TA clone
    Sequence

    Experimental Data
    1. Length of PCR product and PCR efficiency 2. BioReady Taq in Real-Time PCR

    200812925330710

    BioReady Hot Start Taq pac

    The BioReady Hot Start Taq is a developed method to minimize the side effects during PCR. The BioReady Hot Start Taq inhibits Taq polymerase activity during RCP reaction preparation. By limiting Taq polymerase activity at low temperature prior to PCR cycling, non-specific amplification and primer-dimers are reduced.
    Features
    High-Specificity
    High activity
    The PCR product with this enzyme is available to run TA clone directly.
    The product is suitable for realtime PCR reaction owing to 5’-3’ exonuclease activity.
    Application
    PCR reaction
    TA clone
    Sequence
    Experimental Data

    200812925427413

    BioReady Pfu pac.

    BioReady Pfu is a thermostable enzyme of approximately 92 KD isolated from pyrococcus furiosus. BioReady Pfu catalyzes the DNA-dependent polymerization of nucleotide into duplex DNA in the 5’-3’ direction in the presence of magnesium ions. The enzyme also exhibits 3’-5’ exonuclease (proofreading) activity. The base misinsertions that may occur infrequently during polymerization are rapidly emendated by the proofreading activity of the polymerase. In generally, BioReady Pfu is mainly used for polymerization reactions requiring high fidelity synthesis.
    Features
    High fidelity
    Good thermostable
    High activity
    5’-3’ exonuclease activity
    Application
    PCR reaction
    Sequence

    Experimental Data
    1. Electrophoresis result

    200812925528625

    2. Sequence result

    200812925612151

    RT 007 AMV Reverse Transcriptase

    RT007 AMV reverse transcriptase was isolated from avian myeloblastosis virus (AMV) as the holoenzyme of molecular weight 157,000 daltons, using a modification of the method described by houts et al. this preparation is free of nuclease. It is qualified for cDNA synthesis and also for dideoxy sequencing of DNA and RNA. total RNA or poly a+ RNA can be used as template, and optimal temperature is 42℃~-55℃, up to 60 ℃. if nappi is used, 37-41℃ is preferred. RT reaction buffer covered by patents can be used either as 5x /10x stock depending on different purpose (details in standard application). up to 10-12kb cDNA may be obtained according to standard application.

    Features:
    high-sensitivity
    high-yield
    patented rt reaction buffer is suitable for many kinds of applications
    up to 10-12kb cdna
    up to 60 ℃ reaction temperature effectively opens the secondary structure of rna.

    Experimental data

    ①1μg hela total rna was reverse transcripted by rt007 amv at 42℃, 50℃, 55℃ and 60℃, then to run pcr. this is the electrophoresis result.

    ②1μg hela total rna was reverse transcripted by rt007 amv, to run pcr. this is the electrophoresis result of different pcr products. amv reverse transcriptase can reverse transcript mrna up to 9.6kb

     

    200812925735519

    Red Blood Cell Lysis Buffer

    Red blood cell lysis buffer was developed to lyse red blood cells from human whole blood. the buffer provides a very simple, fast economic way to isolate white blood cells from whole blood without destroying the white blood cells. In general, with the buffer, you can get 80% or more white blood cells from the sample.
    Features
    Lysis rapidly and adequately
    The isolated wbc (white blood cells) are very pure.
    The yielded wbc have good biology activity and are beneficial for downstream experiments.
    The isolated wbc can be applied to nucleic acid extraction, pcr and so on.
    The yielded products can be directly applied to the flow cytometer and the purity of cell is high.

    200812925852108

    Lymphocytes Separation Medium

    Lymphocytes Separation Medium uses a density grads principle to isolate lymphocytes (including monocytes). The media is used to isolate lymphocytes and monocytes from human peripheral blood. In general, with the buffer, you can get 70% or more lymphocytes and monocytes from the sample.
    Features:
    The isolated lymphocytes have high purity;
    The operation is convenient and time-saving;
    Using high quality Meglumine Diatri-zoate to make Lymphocytes Separation Medium, the separated monocytes has a high purity and yield.
    Procedure

    2008129301634

    Application
    Lymphocytes Separation Media can be used to isolate lymphocytes and
    monocytes from 1ml~ 5 ml human peripheral bloods. Yielded intact lymphocytes and
    monocytes for further use.
    This media is not intended to deal with the whole bloods in animal’s species.

    Pheol

    Due to the corrosive nature of the phenol, preparation of phenol solution for use in a molecular biology laboratory is a time-consuming and ofen hazardous procedure. Frequently it is necessary to redistill the phenol prior to use to remove contaminants and oxidation products that can damage nucleic acids. In some cases, the PH phenol solutions needs to be adjusted before use. Safety precautions such as protective eyewear, gloves, and a fume hood are necessary when you use the product.
    Features
    We have a full line of products such as equilibrated phenol, mixed phenol, saturated phenol and so on.
    Manufactured under the standard of ISO9001 and strict quality control
    It can be applied for RNA and DNA extraction and there is no contamination of RNase, DNase enzyme
    We provide convenient, safe and multiple types of phenol solutions to customers to avoid many complicated and dangers operations.
    Procedure
    Efficient extraction of cell extracts or solutions containing nucleic acids are most often performed with a series of phenol and phenol-chloroform extractions at a specific pH. Both phenol and chloroform cause proteins to become denatured and become soluble in the organic phase or interphase, while nucleic acids remain in the aqueous phase. After centrifugation, the aqueous phase containing nucleic acids is re-extracted with an equal volume of chloroform-isoamly alcohol. This combination of extractions is to reduce the loss of RNA due to the formation of insoluble protein: RNA complexes at the interphase.
    Chloroform is mixed with phenol to increase the efficiency of nucleic acid extractions by reducing losses of RNA at the interphase. The increased efficiency is due to chloroform’s ability to denature proteins and aid in the removal of lipids, thus improving separation of nucleic acid into the aqueous phase. Phase separation is also enhanced, which assists in the removal of the aqueous phase with minimal cross contamination from the organic phase. In general, isoamyl alcohol is added to phenol-chloroform to reduce foaming.

    20081293047487