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    Articles

    Magnetic Purification Series

    MagaBio General Genomic DNA Purification Kit

    Magabio general genomic DNA purification kit provides a very simple, fast and cost-effective technique to isolate high quality dna. using one simple protocol, high yield of purified dna can be isolated from various sources including whole blood, buffy coat, leukocytes and cultured cells. magabio sample processing is based on proprietary magnetizable particles--magabio. the pure dna can be applied extensively in pcr, sequencing, southern hybridization, mutant analysis, snp and the others.
    According to the special interaction, use magabio nucleic acid separation system with a general protocol---sample processing, magabio adsorption, washing and elution, and can go high-throughput.
    Features
    first elution can acquire >85%
    no organic and high salt solvent
    suitable for automatic platform
    simple, effective and safe process, no centrifugation
    especially used for mini-quantity sample, for example with the volume less than 5mg tissue or 50ul blood
    experimental data

    ww200812921010924

    MagaBio Whole Blood Genomic DNA Purification Kit

    The magabio whole blood genomic DNA purification kit provides a very simple, fast and effective technique to isolate high quality dna. by one simple protocol, purified dna in high yield can be isolated from various sources including whole blood, leukocytes cells. magabio sample processing is based on proprietary magnetizable particles--magabio. the pure dna can be applied extensively in pcr, sequencing, southern hybridization, mutant analysis, snp and the others.
    According to the special interaction, use magabio nucleic acid separation system with a general protocol---sample processing, magabio adsorption, washing and elution, and can go high-throughput.dna in the sample is liberated using protease k (pk) and lysis buffer. released dna is bound exclusively and specifically to the magabio reagent in presence of binding buffer. the dna bound to magabio particles is captured by the magnet and contaminants are removed by washing with wash buffer once or more. the dna is then eluted from the particles with elution buffer or molecular grade water.
    Features:
    special for whole blood sample
    no organic solvent and other toxic reagent
    suitable for automatic platform
    simple, effective and safe process, no centrifugation
    especially used for mini-quantity sample, for example with the volume less than 50ul blood.

    qq200812921329518

    Magabio Plant Genomic DNA Purification Kit

    The magabio plant genomic dna purification kit provides a very simple, fast and effective technique to isolate high quality dna. by one simple protocol, purified dna in high yield can be isolated from various sources including maize, tea, rice, onion and petunia. magabio sample processing is based on proprietary magnetizable particles--magabio. the pure dna can be applied extensively in pcr, sequencing, southern hybridization, mutant analysis, snp and the others.
    According to the special interaction, use magabio nucleic acid separation system with a general protocol---sample processing, magabio adsorption, washing and elution, and can go high-throughput.dna in the sample is liberated using protease k (pk) and lysis buffer. released dna is bound exclusively and specifically to the magabio reagent in presence of binding buffer. the dna bound to magabio particles is captured by the magnet and contaminants are removed by washing with wash buffer once or more. the dna is then eluted from the particles with elution buffer or molecular grade water.
    Features:
    suitable for most of plant sample
    no organic solvent and other toxic reagent
    suitable for automatic platform
    simple, effective and safe process, no centrifugation
    excellent performance for sample with plenty of polysaccharide and polyphenol
    Experimental data

    aa200812921422591

    MagaBio Plasmid DNA Purification Kit

    The magabio plasmid dna purification kit provides a very simple, fast and effective technique to isolate high quality dna. by one simple protocol, purified dna in high yield can be isolated from bacterial. magabio sample processing is based on proprietary magnetizable particles--magabio. the pure dna can be applied extensively in pcr, sequencing, southern hybridization, mutant analysis, snp and the others. according to the special interaction, use magabio nucleic acid separation system with a general protocol---sample processing, magabio adsorption, washing and elution, and can go high-throughput.
    Features:
    suitable for most of bacterial sample with plasmid
    no organic solvent and other toxic reagent
    suitable for automatic platform
    simple, effective and safe process
    excellent result for low copy number plasmid

    Experimental data:

    zz200812921523589

    MagaBio Virus DNA Purification Kit

    The magabio virsus dna purification kit provides a very simple, fast and effective technique to isolate high quality dna. by one simple protocol, purified dna in high yield can be isolated from serum or plasma. magabio sample processing is based on proprietary magnetizable particles--magabio. the pure dna can be applied extensively in pcr, sequencing, southern hybridization, mutant analysis, snp and the others.
    the kit is adapted to purify virus dna from serum or plasma, it can wipe off anticoagulant, especially for serum or plasma stored in edta, citrate, or heparin anticoagulants and won’t affect the downstream experiments, which the pure dna can be used directly.
    Features:
    no organic solvent and other toxic reagent
    suitable for automatic platform
    simple, effective and safe process
    high sensitivity
    experimental data:
    use the kit to purify hbv dna from 100μl hbv positive serum (102-108copies/ml), and then make a program of real-time pcr with 4μl template, the results as follows fig:

    ee200812921628685

    MagaBio Total RNA Purification Kit

    The magabio total RNA purification kit provides a very simple, fast and effective technique to isolate high quality rna. by one simple protocol, purified rna in high yield can be isolated from various sources including whole blood, leukocytes and tissue. magabio sample processing is based on proprietary magnetizable particles--magabio. the pure rna can be applied extensively in rt-pcr, northern hybridization, mutant analysis, rnai and the others.
    features:
    suitable for most of sample, excellent adaptability
    no organic solvent and other toxic reagent
    suitable for automatic platform
    simple, effective and safe process
    especially used for mini-quantity sample, the volume less than 5 mg tissue or 50ul blood

    procedure:

    pro20081292183314