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    Articles

    LineGene I

    LineGene I LineGene I is a complete Real-time PCR detection system that can detect and quantitate nucleic acid sequences by monitoring fluorescence value during the PCR process. With innovative design on hardware and software, the unit is an ideal tool for research use in the field of gene analysis.

    Features
    •  Long life blue led light source
    •  Extremely sensitive pmt detection module
    •  Sandwich thermo-electronic (te) module
    •  Easy yet powerful software
    •  Data export

    Multiple applications

    Detection Format

    Detection Channel

    Reporter Dye

    Simple probe

    530 nm

    Fluorescein

    Taqman format

    530 nm

    FAM

    Sybr Green i

    530 nm

    Sybr Green i

    Hybridization probe

    640 nm

    710 nm

    LC 640 
    LC 705

     

    Hardware

    •  Thermal-electronic (te, peltier) module
    The special designed sandwich te module fits both sides of the sample block well so that can deliver the temperature rapidly and reduce the vertical temperature gradient across different sample blocks, which ensures accurate controlling of pcr reaction temperature.

    •  Blue led light source with a sensitive pmt detection module
    The long life blue led light source doesn't need maintenance or preheating. And the sensitive photomultiplier tube (pmt) offers high sensitivity, broad dynamic range and low crosstalk. With the innovative design to put the light source and the detection module on the bottom of the sample tube, the measurement to the fluorescence is extremely sensitive. 

    •  Use of hot-lid realizes oil free operation

    High sensitivity and accuracy
    By adopting coaxial optical fiber conducting technique, it reduces the interference between excitation light and acceptance light and also increases the ratio between signals and noises so as to improve the accuracy of ct value. the following graph is the amplification result of templates with 5,000 and 10,000 copy numbers on line-gene . 

     

    Software

    Absolute quantification
    After quantitative analysis for the standards, the instrument will get a standard curve related to ct value and copy number. Combining standard curve with the CT value of unknown samples, the instrument can count the original copy number of unknown samples by quantitative analysis. Line-gene has a wide linear detecting range, which can detect  copies. The graph above is a result of a series of standards amplified by LineGene . the standards are seven(7) grades of gradient diluted egf samples with known concentration. the probe is labeled with fam. and the graph below is the standard curve that instrument gets. 

    Relative quantification
    When doing the relative quantification, the instrument doesn't make the standard curve, however, it offers the relative expression level of samples by comparing to inner standards. The relative quantitative detection procedure and result analysis are all finished automatically by software of LineGene. 

    SNPS detection
    LineGene system is a multicolor fluorescence detection system and can carry out the detection analysis of snps.

    Meltin curve analysis
    LineGene system can do melting curve analysis and finish such experiments as qualifying, identifying and mutation analysis.

    Program setting - easy operation
    The program setup interface offers easy operation of program setting and data presentation.

    Program running - intuitive process
    This interface offers the intuitive graphs so to monitor the PCR process Real-time.

    Powerful analyzing functions
    The analysis interface offers easy yet powerful functions for data analysis. It provides many automatic analyzing options, including quantitative analysis and melt-curve analysis.

     

     

    Model

    FQD-33A

    Sample capacity

    33 x 0.2 ml

    Dynamic range

      copies

    Sample volume rang

    10-100μl

    Excitation light

    470nm

    Wavelength, detecting

    530nm, 640nm, 710nm

    Detecting fluorescence

    fam, sybr greenⅰ,lc705, lc640

    Temp.range

    30–99 °c

    Heating rate (max)

    ≥ 4.0°c /sec

    Cooling rate (max.)

    ≥ 4.0°c /sec

    Block temp. uniformity

    ≤ ±0.4 °c

    Temp. accuracy

    ≤ ±0.3°c

    Temp. range of hot lid 105°c

    Power supply

    1. 50HZ  800W

    Size (mm)

    514 x 456 x 245(L x W x H)

    Operating system

    Windows 2000/XP

    Net weight

    23 KG (without computer)