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    Articles

    Infectious diseases: HIV Ag/Ab

    hivThe HIV Ag/Ab assay (Ref. 790001 and 790005) is a 4th generation solid phase Enzyme-Linked ImmunoSorbent Assay using a mixture of antigens and antibodies for the in vitro diagnostic kits screening in human serum or plasma of antibodies to HIV-1, HIV-2 and HIV-P24 antigen.

    Acquired immune deficiency syndrome (AIDS) is a set of symptoms resulting from the incapacitation of the human immune system caused by the Human Immunodeficiency Virus (HIV). HIV infection may progress to a symptomatic phase that is characterized by opportunistic infections and that may cause death.

    The etiological agent of AIDS, HIV, targets specific types of T cells causing Lymphopenia and affecting T cell mediated immunity. HIV is a member of a retrovirus family with two sub-families: HIV-1 and HIV-2. HIV-1 is more virulent and transmittable than HIV-2. HIV-1 is the cause of HIV infections globally, whereas HIV-2 is found predominantly in the countries of West-Africa. As serological cross-reaction between HIV-1 and HIV-2 is highly variable and dependent on the tested sample, antigens for the specific detection of both HIV-1 and HIV-2 are included in the assay. HIV is transmitted through sexual contact with infected persons, sharing needles and syringes with infected people and transfusion of contaminated blood. Enzyme-Linked ImmunoSorbent Assays (such as the HIV Ag/Ab ELISA 4th gen) are recommended for screening human blood and plasma for the presence of anti-HIV antibodies and HIV-P24 antigen.

    • Sensitivity: < 10 pg/ml of HIV-1 P24 Standard; < 1 U/ml of NIBSC International Standard
    • Incubation time: 60' + 30' + 30' + 30'
    • Available format: 192T (Ref. 790001) + 480T (Ref. 790005); or on demand

    The ELISA kits offered are validated on open ELISA automates such as the Dynex Instruments and the Triturus automate.

     

    pdf  Box insert HIV Ag/Ab ELISA - 192T (Ref. 790001) 

    pdf  Box insert HIV Ag/Ab ELISA - 480T (Ref. 790005)  

    pdf  Brochure HIV Ag/Ab ELISA